R G Rutledge
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Explore the profile of R G Rutledge including associated specialties, affiliations and a list of published articles.
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20
Citations
430
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Recent Articles
1.
Tian L, Charest P, Seguin A, Rutledge R
Plant Cell Rep
. 2019 Feb;
19(4):358-362.
PMID: 30754787
Using particle bombardment of mature somatic embryos followed by the induction of secondary embryogenesis in the presence of hygromycin, we produced over 90 lines of transgenic embryonal masses expressing β-glucuronidase...
2.
Rutledge R, Stewart D, Overton C, Klimaszewska K
PLoS One
. 2017 Oct;
12(10):e0185015.
PMID: 28968421
Within a plantation of clonal somatic embryo-derived white spruce trees that belonged to four genotypes, one genotype (G6) has consistently responded for the last 16 years, to the induction of...
3.
Garcia-Mendiguren O, Montalban I, Stewart D, Moncalean P, Klimaszewska K, Rutledge R
PLoS One
. 2015 Jun;
10(6):e0128679.
PMID: 26039876
Background: Although somatic embryogenesis has an unprecedented potential for large-scale clonal propagation of conifers, the ability to efficiently induce the embryonal cultures required for somatic embryo production has long been...
4.
Rutledge R
Nucleic Acids Res
. 2004 Dec;
32(22):e178.
PMID: 15601990
Quantitative real-time PCR has revolutionized many aspects of genetic research, biomedical diagnostics and pathogen detection. Nevertheless, the full potential of this technology has yet to be realized, primarily due to...
5.
Xue B, Charest P, Devantier Y, Rutledge R
Mol Genet Genomics
. 2003 Aug;
270(1):78-86.
PMID: 12920576
PCR amplification with degenerate primers targeted to highly conserved amino acid motifs within the MYB domain was used to demonstrate that black spruce (Picea mariana) possesses a diverse MYB gene...
6.
Rutledge R, Cote C
Nucleic Acids Res
. 2003 Aug;
31(16):e93.
PMID: 12907745
Fluorescent monitoring of DNA amplification is the basis of real-time PCR, from which target DNA concentration can be determined from the fractional cycle at which a threshold amount of amplicon...
7.
Klimaszewska K, Lachance D, Bernier-Cardou M, Rutledge R
Plant Cell Rep
. 2003 Jul;
21(11):1080-7.
PMID: 12836002
Seventy transgenic tissue lines (translines) of three spruce species ( Picea mariana, P. glauca and P. abies) were characterized with respect to the integration pattern of the gus (beta-glucuronidase) gene,...
8.
Cote C, Rutledge R
Plant Cell Rep
. 2003 Jun;
21(6):619-24.
PMID: 12789439
Despite the success of the MUG fluorometric assay for quantitative analysis of beta-glucuronidase (GUS) activity within a vast array of transgenic plant species and tissues, attempts to apply this protocol...
9.
Marshall J, Rutledge R, Blumwald E, Dumbroff E
Tree Physiol
. 2003 Mar;
20(10):701-707.
PMID: 12651520
Significant reductions in needle water content were observed in white spruce (Picea glauca (Moench) Voss), black spruce (Picea mariana (Mill) B.S.P.), and jack pine (Pinus banksiana Lamb.) seedlings in response...
10.
Richard S, LaPointe G, Rutledge R, Seguin A
Plant Cell Physiol
. 2000 Oct;
41(8):982-7.
PMID: 11038059
The phenylpropanoid pathway has important functions in angiospermous plants following exposure to environmental stresses, such as wounding and pathogen attack, that lead to production of compounds including lignin, flavonoids and...