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R A Consigli

Explore the profile of R A Consigli including associated specialties, affiliations and a list of published articles. Areas
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Articles 121
Citations 1069
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Recent Articles
1.
An K, Paulsen A, Tilley M, Consigli R
J Virol Methods . 2000 Sep; 90(1):91-7. PMID: 11011085
Murine polyomavirus major structural protein VP1 could assemble into capsid-like particles when expressed in the baculovirus system. The recombinant capsid-like particles that were purified by CsCl density gradient centrifugation were...
2.
An K, Fattaey H, Paulsen A, Consigli R
Virus Res . 2000 Apr; 67(1):81-90. PMID: 10773321
The current study was developed to determine if polyomavirus infected 3T6 mouse cells evoked an apoptotic or a necrotic mechanism during infection. Infected cells were analyzed by flow cytometry, transmission...
3.
An K, Lovgren T, Tilley M, Consigli R
J Virol Methods . 2000 Feb; 84(2):153-9. PMID: 10680964
The structural protein genes of polyomavirus were expressed in the baculovirus system, and the proteins were found to assemble into capsid-like particles capable of packaging insect cell DNA. Recombinant capsid-like...
4.
An K, Smiley S, Gillock E, Reeves W, Consigli R
Virus Res . 1999 Oct; 64(2):173-85. PMID: 10518713
The baculovirus system was used to construct and isolate AcMNPV-VP1, AcMNPV-VP2 and AcMNPV-VP3 recombinant viruses which express the respective avian polyomavirus (APV) structural proteins in Sf9 insect cells. These recombinant...
5.
An K, Gillock E, Sweat J, Reeves W, Consigli R
J Gen Virol . 1999 Apr; 80 ( Pt 4):1009-1016. PMID: 10211971
The genes encoding the structural proteins (VP1, VP2 and VP3) of murine polyomavirus were cloned into the p2Bac dual multiple cloning site vector, individually or jointly, and the corresponding proteins...
6.
Gillock E, An K, Consigli R
Virus Res . 1999 Jan; 58(1-2):149-60. PMID: 9879771
Using the pBlueBacIII baculovirus transfer vector, N11-VP1, a truncated form of the polyomavirus major capsid protein VP1, was cloned for expression in the baculovirus-insect cell expression system. The N11-VP1 protein...
7.
Gillock E, Rottinghaus S, Chang D, Cai X, Smiley S, An K, et al.
J Virol . 1997 Apr; 71(4):2857-65. PMID: 9060642
Using the p2Bac dual multiple cloning site transfer vector, the polyomavirus major capsid protein gene VP1 was cloned for expression in the baculovirus-insect cell expression system. The 5-day-infected cellular lysate...
8.
Smiley S, Gillock E, Black M, Consigli R
Exp Cell Res . 1997 Feb; 230(2):411-4. PMID: 9024803
The hybridoma cell line, 3G10G5, producing a monoclonal antibody to the major capsid protein VP1 from the avian polyomavirus budgerigar fledgling disease virus, was produced from a Balb/C mouse. This...
9.
Rodgers R, Consigli R
Virus Res . 1996 Oct; 44(2):123-35. PMID: 8879141
Calcium ions appear to play a major role in maintaining the structural integrity and assembly of papovavirus virions and are likely involved in the process of viral uncoating. Recently it...
10.
Burton K, Consigli R
Virus Res . 1996 Feb; 40(2):141-7. PMID: 8725110
Polyomavirus VP1 has been shown to be modified by phosphorylation, sulfation, acetylation and hydroxylation. The major capsid protein VP1 is now shown to be modified by methylation. Addition of cycloheximide...