P M Giffard
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Explore the profile of P M Giffard including associated specialties, affiliations and a list of published articles.
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28
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391
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Recent Articles
11.
Hafner G, Yang I, Wolter L, Stafford M, Giffard P
Biotechniques
. 2001 Apr;
30(4):852-6, 858, 860 passim.
PMID: 11314268
We have demonstrated the isothermal in vitro amplification and multimerization of several different linear DNA targets using only two primers and the strongly strand-displacing exonuclease-negative Bst DNA polymerase. This reaction...
12.
Turner M, Giffard P
Infect Immun
. 1999 Sep;
67(10):5486-9.
PMID: 10496936
The basic surface protein, BspA, has been used as a fusion partner to direct peptide antigens from the human immunodeficiency virus gp41 protein and the Chlamydia psittaci OmpA protein to...
13.
Turner M, Woodberry T, Hafner L, Giffard P
J Bacteriol
. 1999 Mar;
181(7):2192-8.
PMID: 10094698
BspA is a basic surface-exposed protein from Lactobacillus fermentum BR11. Sequence comparisons have shown that it is a member of family III of the solute binding proteins. It is 89%...
14.
Turner M, Timms P, Hafner L, Giffard P
J Bacteriol
. 1997 May;
179(10):3310-6.
PMID: 9150229
Extraction of Lactobacillus fermentum BR11 cells with 5 M LiCl yielded a preparation containing a single predominant polypeptide with an apparent molecular mass of 32 kDa. A clone encoding an...
15.
Norman J, Giffard P
Arch Oral Biol
. 1996 Jun;
41(6):565-70.
PMID: 8937647
The Actinomyces naeslundii T14V gene levJ encodes a sucrase with fructanase activity and may be responsible for the fructanase activity observed bound to the surface of A. naeslundii T14V cells....
16.
Simpson C, Giffard P, Jacques N
Infect Immun
. 1995 Feb;
63(2):609-21.
PMID: 7822030
Fractionation of the culture medium showed that Streptococcus salivarius ATCC 25975 secreted a glucosyltransferase (Gtf) that was primer independent. On the basis of this observation, a gene library of S....
17.
Norman J, Bunny K, Giffard P
Gene
. 1995 Jan;
152(1):93-8.
PMID: 7828936
A library of Actinomyces naeslundii T14V DNA was constructed in plasmid pUC18 and from this several sucrose-positive clones were isolated. Evidence was obtained that all these clones contained the same...
18.
Giffard P, Jacques N
J Dent Res
. 1994 Jun;
73(6):1133-41.
PMID: 8046101
The C-termini of the glucosyltransferases (Gtfs) of oral streptococci are responsible for glucan binding. These glucan-binding domains (GBDs) are composed of a series of repeated sequences that have been classified...
19.
Giffard P, Allen D, Milward C, Simpson C, Jacques N
J Gen Microbiol
. 1993 Jul;
139(7):1511-22.
PMID: 8371114
Many strains of oral streptococci secrete glucosyltransferases (GTFs) that polymerize sucrose into glucans that form an integral part of the plaque matrix on the tooth surface. Recently, we reported the...
20.
Rathsam C, Giffard P, Jacques N
J Bacteriol
. 1993 Jul;
175(14):4520-7.
PMID: 8331080
The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the...