N S Shepherd
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Explore the profile of N S Shepherd including associated specialties, affiliations and a list of published articles.
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18
Citations
276
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Recent Articles
1.
Sternberg N, Shepherd N
Curr Protoc Hum Genet
. 2008 Apr;
Chapter 5:Unit 5.3.
PMID: 18428291
The bacteriophage P1 cloning system was originally developed as an alternative to YAC and cosmid systems for cloning high-molecular-weight genomic DNA. This unit details the preparation of the bacteriophage P1...
2.
Ehm M, Karnoub M, Sakul H, Gottschalk K, Holt D, Weber J, et al.
Am J Hum Genet
. 2000 May;
66(6):1871-81.
PMID: 10793009
Type 2 diabetes is a serious, genetically influenced disease for which no fully effective treatments are available. Identification of biochemical or regulatory pathways involved in the disease syndrome could lead...
3.
Chatterjee P, Yarnall D, Haneline S, Godlevski M, Thornber S, Robinson P, et al.
Proc Natl Acad Sci U S A
. 1999 Nov;
96(23):13276-81.
PMID: 10557311
A loxP-transposon retrofitting strategy for generating large nested deletions from one end of the insert DNA in bacterial artificial chromosomes and P1 artificial chromosomes was described recently [Chatterjee, P. K....
4.
Chyan Y, Ackerman S, Shepherd N, MCBRIDE O, Widen S, Wilson S, et al.
Nucleic Acids Res
. 1994 Jul;
22(14):2719-25.
PMID: 7914364
DNA polymerase beta (beta-pol) is a single-copy gene that is considered to be part of the DNA repair machinery in mammalian cells. Using two human genomic libraries we have cloned...
5.
Shepherd N, Pfrogner B, Coulby J, Ackerman S, Vaidyanathan G, Sauer R, et al.
Proc Natl Acad Sci U S A
. 1994 Mar;
91(7):2629-33.
PMID: 8146166
We describe here the construction and initial characterization of a 3-fold coverage genomic library of the human haploid genome that was prepared using the bacteriophage P1 cloning system. The cloned...
6.
Shepherd N, Smoller D
Genet Eng (N Y)
. 1994 Jan;
16:213-28.
PMID: 7765198
In retrospect, it is remarkable how swiftly the P1 cloning system has progressed in only a few years from a novel cloning system to one now widely used for the...
7.
Zhang X, Lee K, Heng H, Tsui L, Parnes J, Shepherd N, et al.
Genet Anal Tech Appl
. 1994 Jan;
11(5-6):129-39.
PMID: 7710778
The T-lymphocyte coreceptor molecules CD8 (composed of CD8 alpha and CD8 beta chains) and CD4 undergo a complex pattern of regulated expression during T-cell maturation. In the thymus, the most...
8.
Shepherd N, Rhoades M, Dempsey E
Dev Genet
. 1989 Jan;
10(6):507-19.
PMID: 2557991
A new allele of the maize A1 gene, a gene required for anthocyanin pigment biosynthesis, was identified in a genetic stock exhibiting a high frequency of chromosome breakage at the...
9.
Brown J, Mattes M, OReilly C, Shepherd N
Mol Gen Genet
. 1989 Jan;
215(2):239-44.
PMID: 2540411
We have molecularly cloned the rDt transposon, one component of the classic "Dotted" two-element system of controlling elements. The rDt transposon was identified as a DNA insertion in each of...
10.
OReilly C, Shepherd N, Pereira A, Schwarz-Sommer Z, Bertram I, Robertson D, et al.
EMBO J
. 1985 Apr;
4(4):877-82.
PMID: 15938046
The a1 locus of Zea mays has been cloned using transposable elements as gene tags. The strategy was to make genomic libraries from maize stocks with a1 mutations induced either...