Mitsuhiro Itaya
Overview
Explore the profile of Mitsuhiro Itaya including associated specialties, affiliations and a list of published articles.
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Articles
72
Citations
778
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Recent Articles
1.
Itaya M
Biosci Biotechnol Biochem
. 2024 Dec;
89(3):347-353.
PMID: 39663205
Cloning of small DNA segments has been established using Escherichia coli plasmids. The cloned DNA can be transferred to various cells using transformation. In contrast, cloning of large DNA segments...
2.
Itaya M, Kataoka M
Genes Cells
. 2024 Apr;
29(7):584-588.
PMID: 38660704
Bacillus subtilis was engineered to produce circular subgenomes that are directly transmittable to another B. subtilis. The conjugational plasmid pLS20 integrated into the B. subtilis genome supported not only subgenome...
3.
Itaya M, Sato M, Watanabe S, Kataoka M
J Biochem
. 2022 Sep;
172(5):313-319.
PMID: 36047835
In this study, a Bacillus natto strain named NEST141 was constructed. The strain carries no plasmids and is an authentic proline auxotroph-a feature that confers effective selection conditions for plasmids...
4.
Itaya M
J Gen Appl Microbiol
. 2022 May;
68(2):45-53.
PMID: 35491108
Bacillus subtilis Marburg 168 is a unique platform for genome engineering and genome synthesis. Genome scale DNA sequences can be synthesized by repeated integration of small DNA segments in the...
5.
Takemura M, Takagi C, Aikawa M, Araki K, Choi S, Itaya M, et al.
Microb Cell Fact
. 2021 Oct;
20(1):194.
PMID: 34627253
Background: Members of the genus Planococcus have been revealed to utilize and degrade solvents such as aromatic hydrocarbons and alkanes, and likely to acquire tolerance to solvents. A yellow marine...
6.
Yokoi T, Itaya M, Mori H, Kataoka M
J Gen Appl Microbiol
. 2019 Jun;
65(5):265-272.
PMID: 31168023
The Gram-positive bacterium Bacillus subtilis plays important roles in both industrial applications and basic research. However, transformation of competent B. subtilis cells is more difficult to achieve compared with that...
7.
Itaya M, Sato M, Watanabe S, Yoshikawa H, Tomita M, Sato R
J Biochem
. 2019 Apr;
166(3):231-236.
PMID: 31004491
Bacillus subtilis 168 has been explored as a platform for the synthesis and transmission of large DNA. Two inherent DNA incorporation systems, natural transformation and pLS20-based conjugation transfer, enable rapid...
8.
Itaya M, Nagasaku M, Shimada T, Ohtani N, Shiwa Y, Yoshikawa H, et al.
FEMS Microbiol Lett
. 2019 Feb;
366(4).
PMID: 30726909
Bacillus subtilis (natto) is generally regarded as a safe bacterium and used as a host for the production of several materials. However, genetic engineering of B. subtilis (natto) is not...
9.
Itaya M, Sato M, Hasegawa M, Kono N, Tomita M, Kaneko S
Sci Rep
. 2018 Jun;
8(1):8792.
PMID: 29884789
Bacillus subtilis offers a platform for giant DNA synthesis, which is mediated by the connection of overlapping DNA segments called domino DNA, in the cloning locus of the host. The...
10.
Itaya M, Kusakabe H, Sato M, Tomita M, Sato R
J Biochem
. 2018 Feb;
164(1):15-20.
PMID: 29420737
Synechococcus elongatus PCC7942, a cyanobacterium that uses light and carbon dioxide to grow, has a high ability to incorporate DNA by transformation. To assess the effective delivery of large DNA...