Michael Marko
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Explore the profile of Michael Marko including associated specialties, affiliations and a list of published articles.
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Recent Articles
1.
Adams R, Liu Z, Hsieh C, Marko M, Lederer W, Jafri M, et al.
Curr Issues Mol Biol
. 2023 Jul;
45(7):6097-6115.
PMID: 37504301
Mitochondria in mammalian cardiomyocytes display considerable structural heterogeneity, the significance of which is not currently understood. We use electron microscopic tomography to analyze a dataset of 68 mitochondrial subvolumes to...
2.
Summers R, Morrill J, Leith A, Marko M, Piston D, Stonebraker A
Dev Growth Differ
. 2023 Jun;
35(1):41-57.
PMID: 37280928
Fourth cleavage of the sea urchin embryo produces 16 blastomeres that are the starting point for analyses of cell lineages and bilateral symmetry. We used optical sectioning, scanning electron microscopy...
3.
Obermair M, Hettler S, Hsieh C, Dries M, Marko M, Gerthsen D
Ultramicroscopy
. 2020 Aug;
218:113086.
PMID: 32781400
Phase plates (PPs) are beneficial devices to improve the phase contrast of life-science objects in cryo-transmission electron microscopy (TEM). The development of the hole-free (HF) PP, which consists of a...
4.
He J, Hsieh C, Wu Y, Schmelzer T, Wang P, Lin Y, et al.
J Struct Biol
. 2017 Jun;
199(2):114-119.
PMID: 28559166
Cryo-electron tomography (cryo-ET) is a well-established technique for studying 3D structural details of subcellular macromolecular complexes and organelles in their nearly native context in the cell. A primary limitation of...
5.
Marko M, Hsieh C, Leith E, Mastronarde D, Motoki S
Microsc Microanal
. 2016 Nov;
22(6):1316-1328.
PMID: 27881198
Phase plate (PP) imaging has proven to be valuable in transmission cryo electron microscopy of unstained, native-state biological specimens. Many PP types have been described, however until the recent implementation...
6.
Kishchenko G, Danev R, Fisher R, He J, Hsieh C, Marko M, et al.
J Struct Biol
. 2015 Jul;
191(3):299-305.
PMID: 26210582
Zernike phase-plate (ZPP) imaging greatly increases contrast in cryo-electron microscopy, however fringe artifacts appear in the images. A computational de-fringing method has been proposed, but it has not been widely...
7.
Wagenknecht T, Hsieh C, Marko M
Eur J Transl Myol
. 2015 Jun;
25(1):4823.
PMID: 26052414
Cryo-electron tomography (cryo-ET) has emerged as perhaps the only practical technique for revealing nanometer-level three-dimensional structural details of subcellular macromolecular complexes in their native context, inside the cell. As currently...
8.
Hsieh C, Schmelzer T, Kishchenko G, Wagenknecht T, Marko M
J Struct Biol
. 2013 Nov;
185(1):32-41.
PMID: 24211822
Vitreous freezing offers a way to study cells and tissue in a near-native state by cryo-transmission electron microscopy (cryo-TEM), which is important when structural information at the macromolecular level is...
9.
Marko M, Meng X, Hsieh C, Roussie J, Striemer C
J Struct Biol
. 2013 Sep;
184(2):237-44.
PMID: 23994351
Imaging with Zernike phase plates is increasingly being used in cryo-TEM tomography and cryo-EM single-particle applications. However, rapid ageing of the phase plates, together with the cost and effort in...
10.
Forbes S, Martinelli D, Hsieh C, Ault J, Marko M, Mannella C, et al.
Infect Immun
. 2012 Apr;
80(7):2454-63.
PMID: 22473607
Invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is an energetically demanding process, involving the transfer of effector proteins from invading bacteria into host cells via a specialized...