Matthew J Guille
Overview
Explore the profile of Matthew J Guille including associated specialties, affiliations and a list of published articles.
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Articles
13
Citations
125
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Recent Articles
1.
Noble A, Abu-Daya A, Guille M
Cold Spring Harb Protoc
. 2022 Feb;
2022(6):Pdb.prot107011.
PMID: 35135888
Transgenic frogs can be very efficiently generated using I-SceI meganuclease, a nuclease with an 18-bp recognition site. The desired transgene must be flanked by I-SceI sites, in either a plasmid...
2.
Martin S, Page S, Piccinni M, Guille M
Cold Spring Harb Protoc
. 2020 Oct;
2020(12).
PMID: 33037077
Verifying that a new antibody recognizes its target can be difficult. In this protocol, expression of a target protein in embryos is either knocked down using CRISPR-Cas9 technology (for zygotic...
3.
Piccinni M, Guille M
Cold Spring Harb Protoc
. 2020 Jan;
2020(9):105585.
PMID: 31900323
For work in , frog-specific antibodies must usually be raised, although a few antibodies against mammalian proteins cross-react. To produce an immunogen for antibody production, human embryonic kidney (HEK) expression...
4.
Piccinni M, Guille M
Cold Spring Harb Protoc
. 2020 Jan;
2020(9):105619.
PMID: 31900322
Antibody production for work in involves the immunization of a host with an antigen, usually a protein or peptide alien to the host. The antibody-containing serum, normally returned to the...
5.
Piccinni M, Guille M
Cold Spring Harb Protoc
. 2020 Jan;
2020(9):105593.
PMID: 31900321
Frog-specific antibodies usually must be raised for work in Selecting a host animal whose immune system will respond to a target antigen with an antibody response is essential to obtaining...
6.
Tinsley R, Coxhead P, Stott L, Tinsley M, Piccinni M, Guille M
Biol Conserv
. 2015 Apr;
184:380-388.
PMID: 25843959
The chytrid fungus () is notorious amongst current conservation biology challenges, responsible for mass mortality and extinction of amphibian species. World trade in amphibians is implicated in global dissemination. Exports...
7.
Love N, Thuret R, Chen Y, Ishibashi S, Sabherwal N, Paredes R, et al.
Development
. 2011 Nov;
138(24):5451-8.
PMID: 22110059
As studies aim increasingly to understand key, evolutionarily conserved properties of biological systems, the ability to move transgenesis experiments efficiently between organisms becomes essential. DNA constructions used in transgenesis usually...
8.
King A, Cragg S, Li Y, Dymond J, Guille M, Bowles D, et al.
Proc Natl Acad Sci U S A
. 2010 Mar;
107(12):5345-50.
PMID: 20212162
The digestion of lignocellulose is attracting attention both in terms of basic research into its metabolism by microorganisms and animals, and also as a means of converting plant biomass into...
9.
Llewellyn K, Cary P, McClellan J, Guille M, Scarlett G
Biochim Biophys Acta
. 2009 Aug;
1789(11-12):675-80.
PMID: 19665599
We have previously shown that a critical region of the gata2 promoter contains an inverted CCAAT box and adopts a partial A-form DNA structure in vitro. At gastrula stages of...
10.
Zygotic nucleosome assembly protein-like 1 has a specific, non-cell autonomous role in hematopoiesis
Abu-Daya A, Steer W, Trollope A, Friedeberg C, Patient R, Thorne A, et al.
Blood
. 2005 Apr;
106(2):514-20.
PMID: 15811954
Nucleosome assembly proteins (NAPs) bind core histones, facilitate chromatin remodeling, and can act as transcriptional coactivators. We previously described the isolation of a Xenopus NAP1-like (xNAP1L) cDNA, which encodes a...