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Mary D Barkley

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Articles 15
Citations 294
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Recent Articles
1.
Pan C, Muino P, Barkley M, Callis P
J Phys Chem B . 2011 Mar; 115(12):3245-53. PMID: 21370844
Tryptophan (Trp) fluorescence is potentially a powerful probe for studying the conformational ensembles of proteins in solution, as it is highly sensitive to the local electrostatic environment of the indole...
2.
Seckler J, Barkley M, Wintrode P
Biophys J . 2010 Dec; 100(1):144-53. PMID: 21190666
Efavirenz is a second-generation nonnucleoside reverse transcriptase inhibitor (NNRTI) and a common component of clinically approved anti-AIDS regimens. NNRTIs are noncompetitive inhibitors that bind in a hydrophobic pocket in the...
3.
Braz V, Barkley M, Jockusch R, Wintrode P
Biochemistry . 2010 Nov; 49(49):10565-73. PMID: 21090588
Efavirenz (EFV) is a potent nonnucleoside reverse transcriptase inhibitor (NNRTI) used in the treatment of AIDS. NNRTIs bind in a hydrophobic pocket located in the p66 subunit of reverse transcriptase...
4.
Braz V, Holladay L, Barkley M
Biochemistry . 2009 Dec; 49(3):601-10. PMID: 20039714
Efavirenz (EFV) is a nonnucleoside reverse transcriptase inhibitor (NNRTI) of HIV-1 reverse transcriptase (RT) used for the treatment of AIDS. RT is a heterodimer composed of p66 and p51 subunits;...
5.
Venezia C, Meany B, Braz V, Barkley M
Biochemistry . 2009 Sep; 48(38):9084-93. PMID: 19715314
The biologically active form of HIV-1 reverse transcriptase (RT) is the p66/p51 heterodimer. The process of maturation of the heterodimer from precursor proteins is poorly understood. Previous studies indicated that...
6.
Seckler J, Howard K, Barkley M, Wintrode P
Biochemistry . 2009 Jul; 48(32):7646-55. PMID: 19594135
Crystal structures and simulations suggest that conformational changes are critical for the function of HIV-1 reverse transcriptase. The enzyme is an asymmetric heterodimer of two subunits, p66 and p51. The...
7.
Pan C, Callis P, Barkley M
J Phys Chem B . 2006 Mar; 110(13):7009-16. PMID: 16571015
The wavelength of maximum emission of tryptophan depends on the local electrostatic environment of the indole chromophore. The time-resolved emission spectra of seven rigid cyclic hexapeptides containing a single tryptophan...
8.
Venezia C, Howard K, Ignatov M, Holladay L, Barkley M
Biochemistry . 2006 Mar; 45(9):2779-89. PMID: 16503633
Recent studies showed that nonnucleoside reverse transcriptase inhibitors (NNRTIs) have variable effects on dimerization of p66 and p51 subunits of HIV-1 reverse transcriptase (RT). Efavirenz, one of three NNRTIs currently...
9.
Ignatov M, Berdis A, Le Grice S, Barkley M
Biochemistry . 2005 Apr; 44(14):5346-56. PMID: 15807528
Previous pre-steady-state kinetic studies of equine infectious anemia virus-1 (EIAV) reverse transcriptase (RT) showed two effects of DNA substrates containing the central termination sequence (CTS) on the polymerization reaction: reduction...
10.
Pan C, Barkley M
Biophys J . 2004 Jun; 86(6):3828-35. PMID: 15189879
The peptide bond quenches tryptophan fluorescence by excited-state electron transfer, which probably accounts for most of the variation in fluorescence intensity of peptides and proteins. A series of seven peptides...