Marie-Eve Lacombe-Harvey
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Explore the profile of Marie-Eve Lacombe-Harvey including associated specialties, affiliations and a list of published articles.
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7
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94
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Recent Articles
1.
Lacombe-Harvey M, Brzezinski R, Beaulieu C
Appl Microbiol Biotechnol
. 2018 Jun;
102(17):7219-7230.
PMID: 29931600
Actinobacteria, a large group of Gram-positive bacteria, secrete a wide range of extracellular enzymes involved in the degradation of organic compounds and biopolymers including the ubiquitous aminopolysaccharides chitin and chitosan....
2.
Viens P, Lacombe-Harvey M, Brzezinski R
Mar Drugs
. 2015 Oct;
13(11):6566-87.
PMID: 26516868
Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides...
3.
Lacombe-Harvey M, Fortin M, Ohnuma T, Fukamizo T, Letzel T, Brzezinski R
BMC Biochem
. 2013 Sep;
14:23.
PMID: 24041306
Background: Streptomyces sp. N174 chitosanase (CsnN174), a member of glycoside hydrolases family 46, is one of the most extensively studied chitosanases. Previous studies allowed identifying several key residues of this...
4.
Ghinet M, Roy S, Poulin-Laprade D, Lacombe-Harvey M, Morosoli R, Brzezinski R
Biochem Cell Biol
. 2010 Nov;
88(6):907-16.
PMID: 21102653
Chitosan, an N-deacetylated derivative of chitin, has attracted much attention as an antimicrobial agent against fungi, bacteria, and viruses. Chitosanases, the glycoside hydrolases responsible for chitosan depolymerisation, are intensively studied...
5.
Lacombe-Harvey M, Fukamizo T, Gagnon J, Ghinet M, Dennhart N, Letzel T, et al.
FEBS J
. 2009 Jan;
276(3):857-69.
PMID: 19143844
The chitosanase from Streptomyces sp. N174 (CsnN174) is an inverting glycoside hydrolase belonging to family 46. Previous studies identified Asp40 as the general base residue. Mutation of Asp40 into glycine...
6.
Dennhart N, Fukamizo T, Brzezinski R, Lacombe-Harvey M, Letzel T
J Biotechnol
. 2008 Mar;
134(3-4):253-60.
PMID: 18359118
The chitosanase-catalyzed hydrolysis of chitosan oligosaccharides was investigated for the first time by real-time electrospray ionization-mass spectrometry (ESI-MS). As chitosan oligosaccharides (GlcNn, n=2-6) were hydrolyzed by exochitosanase (exo-beta-glucosaminidase) from Amycolatopsis...
7.
Katsumi T, Lacombe-Harvey M, Tremblay H, Brzezinski R, Fukamizo T
Biochem Biophys Res Commun
. 2005 Nov;
338(4):1839-44.
PMID: 16288718
We examined the oligosaccharide binding to Streptomyces sp. N174 chitosanase by fluorescence spectroscopy. By means of the tryptophan fluorescence quenching, the oligosaccharide binding abilities were evaluated using the three mutant...