Margaret A Fuqua
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Explore the profile of Margaret A Fuqua including associated specialties, affiliations and a list of published articles.
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6
Citations
275
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Recent Articles
1.
Gregor B, Coston M, Adams E, Arakaki J, Borensztejn A, Do T, et al.
Nat Protoc
. 2023 Dec;
19(2):565-594.
PMID: 38087082
To produce abundant cell culture samples to generate large, standardized image datasets of human induced pluripotent stem (hiPS) cells, we developed an automated workflow on a Hamilton STAR liquid handler...
2.
Viana M, Chen J, Knijnenburg T, Vasan R, Yan C, Arakaki J, et al.
Nature
. 2023 Jan;
613(7943):345-354.
PMID: 36599983
Understanding how a subset of expressed genes dictates cellular phenotype is a considerable challenge owing to the large numbers of molecules involved, their combinatorics and the plethora of cellular behaviours...
3.
Roberts B, Hendershott M, Arakaki J, Gerbin K, Malik H, Nelson A, et al.
Stem Cell Reports
. 2019 Apr;
12(5):1145-1158.
PMID: 30956114
We describe a multistep method for endogenous tagging of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and a constitutively expressed mCherry...
4.
Haupt A, Grancharova T, Arakaki J, Fuqua M, Roberts B, Gunawardane R
J Vis Exp
. 2018 Sep;
(138).
PMID: 30199041
A protocol is presented for generating human induced pluripotent stem cells (hiPSCs) that express endogenous proteins fused to in-frame N- or C-terminal fluorescent tags. The prokaryotic CRISPR/Cas9 system (clustered regularly...
5.
Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua M, et al.
Mol Biol Cell
. 2017 Aug;
28(21):2854-2874.
PMID: 28814507
We present a CRISPR/Cas9 genome-editing strategy to systematically tag endogenous proteins with fluorescent tags in human induced pluripotent stem cells (hiPSC). To date, we have generated multiple hiPSC lines with...
6.
Yao Z, Mich J, Ku S, Menon V, Krostag A, Martinez R, et al.
Cell Stem Cell
. 2017 Jan;
20(1):120-134.
PMID: 28094016
During human brain development, multiple signaling pathways generate diverse cell types with varied regional identities. Here, we integrate single-cell RNA sequencing and clonal analyses to reveal lineage trees and molecular...