Alpha 2.0
Login Register
» Authors » Lenka Bourova

Lenka Bourova

Explore the profile of Lenka Bourova including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 12
Citations 81
Followers 0
Related Specialties
Neurology
Cell Biology
Biochemistry
Top 10 Co-Authors
Petr Svoboda
Jiri Novotny
Lucie Hejnova
Ivanna Ihnatovych
Jiri Stohr
Pavel Mares
Renata Haugvicova
Tomas Obsil
Miroslava Vosahlikova
Petr Herman
Published In
Brain Res Dev Brain Res
Med Sci Monit
J Neurochem
Biochem J
J Cell Mol Med
Affiliations
Soon will be listed here.
Recent Articles
1.
Long-term adaptation to high doses of morphine causes desensitization of mu-OR- and delta-OR-stimulated G-protein response in forebrain cortex but does not decrease the amount of G-protein alpha subunits
Bourova L, Vosahlikova M, Kagan D, Dlouha K, Novotny J, Svoboda P
Med Sci Monit . 2010 Jul; 16(8):BR260-70. PMID: 20671607
Background: The functional activity of trimeric guanine-nucleotide-binding proteins (G-proteins) represents an essential step in linking and regulation of the opioid receptor (mu-, delta- and kappa-OR)-initiated signaling pathways. Theoretical basis and/or...
2.
14-3-3 protein interacts with and affects the structure of RGS domain of regulator of G protein signaling 3 (RGS3)
Rezabkova L, Boura E, Herman P, Vecer J, Bourova L, Sulc M, et al.
J Struct Biol . 2010 Mar; 170(3):451-61. PMID: 20347994
Regulator of G protein signaling (RGS) proteins function as GTPase-activating proteins (GAPs) for the alpha-subunit of heterotrimeric G proteins. Several RGS proteins have been found to interact with 14-3-3 proteins....
3.
Protein alterations induced by long-term agonist treatment of HEK293 cells expressing thyrotropin-releasing hormone receptor and G11alpha protein
Drastichova Z, Bourova L, Hejnova L, Jedelsky P, Svoboda P, Novotny J
J Cell Biochem . 2009 Nov; 109(1):255-64. PMID: 19911373
This study aimed to determine whether sustained stimulation with thyrotropin-releasing hormone (TRH), a peptide with important physiological functions, can possibly affect expression of plasma membrane proteins in HEK293 cells expressing...
4.
Isolation of plasma membrane compartments from rat brain cortex; detection of agonist-stimulated G protein activity
Bourova L, Stohr J, Lisy V, Rudajev V, Novotny J, Svoboda P
Med Sci Monit . 2009 Apr; 15(4):BR111-22. PMID: 19333192
Background: Heterotrimeric guanine nucleotide-binding proteins (G proteins) play an essential role in linking cell-surface receptors to effector proteins at the plasma membrane. The functional activities of G proteins in various...
5.
Disruption of the plasma membrane integrity by cholesterol depletion impairs effectiveness of TRH receptor-mediated signal transduction via G(q)/G(11)alpha proteins
Ostasov P, Bourova L, Hejnova L, Novotny J, Svoboda P
J Recept Signal Transduct Res . 2007 Dec; 27(5-6):335-52. PMID: 18097936
We monitored the radioligand-binding characteristics of thyrotropin-releasing hormone (TRH) receptors, functional activity of G(q/11)alpha proteins, and functional status of the whole signaling cascade in HEK293 expressing high levels of TRH...
6.
Modulation of adenylyl cyclase activity in young and adult rat brain cortex. Identification of suramin as a direct inhibitor of adenylyl cyclase
Stohr J, Novotny J, Bourova L, Svoboda P
J Cell Mol Med . 2005 Dec; 9(4):940-52. PMID: 16364201
Adenylyl cyclase (AC) in brain cortex from young (12-day-old) rats exhibits markedly higher activity than in adult (90-day-old) animals. In order to find some possibly different regulatory features of AC...
7.
Increased baclofen-stimulated G protein coupling and deactivation in rat brain cortex during development
Stohr J, Bourova L, Hejnova L, Ihnatovych I, Novotny J, Svoboda P
Brain Res Dev Brain Res . 2004 Jul; 151(1-2):67-73. PMID: 15246693
The number and affinity of GABA(B) receptors (assayed by the specific antagonist [(3)H]CGP54626A) was unchanged when compared in carefully washed cerebrocortical membranes from young (12-day-old) and adult (90-day-old) rats. In...
8.
delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains
Bourova L, Kostrnova A, Hejnova L, Moravcova Z, Moon H, Novotny J, et al.
J Neurochem . 2003 Mar; 85(1):34-49. PMID: 12641725
Low-density membrane fragments (domains) were separated from the bulk of plasma membranes of human embryonic kidney (HEK)293 cells expressing a delta-opioid (DOP) receptor-Gi1alpha fusion protein by drastic homogenization and flotation...
9.
Micromachined nanocalorimetric sensor for ultra-low-volume cell-based assays
Johannessen E, Weaver J, Bourova L, Svoboda P, Cobbold P, Cooper J
Anal Chem . 2002 May; 74(9):2190-7. PMID: 12033326
Current strategies for cell-based screening generally focus on the development of highly specific assays, which require an understanding of the nature of the signaling molecules and cellular pathways involved. In...
10.
Impaired noradrenaline-induced lipolysis in white fat of aP2-Ucp1 transgenic mice is associated with changes in G-protein levels
Flachs P, Novotny J, Baumruk F, Bardova K, Bourova L, Miksik I, et al.
Biochem J . 2002 May; 364(Pt 2):369-76. PMID: 12023879
In vitro experiments suggest that stimulation of lipolysis by catecholamines in adipocytes depends on the energy status of these cells. We tested whether mitochondrial uncoupling proteins (UCPs) that control the...