Kristofer Gryte
Overview
Explore the profile of Kristofer Gryte including associated specialties, affiliations and a list of published articles.
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Articles
8
Citations
239
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0
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Recent Articles
1.
Duchi D, Gryte K, Robb N, Morichaud Z, Sheppard C, Brodolin K, et al.
Nucleic Acids Res
. 2017 Nov;
46(2):677-688.
PMID: 29177430
Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex....
2.
Duchi D, Bauer D, Fernandez L, Evans G, Robb N, Hwang L, et al.
Mol Cell
. 2016 Sep;
63(6):939-50.
PMID: 27618490
In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription...
3.
Crawford R, Torella J, Aigrain L, Plochowietz A, Gryte K, Uphoff S, et al.
Biophys J
. 2013 Dec;
105(11):2439-50.
PMID: 24314075
Studies of biomolecules in vivo are crucial to understand their function in a natural, biological context. One powerful approach involves fusing molecules of interest to fluorescent proteins to study their...
4.
Robb N, Cordes T, Hwang L, Gryte K, Duchi D, D Craggs T, et al.
J Mol Biol
. 2013 Jan;
425(5):875-85.
PMID: 23274143
Bacterial transcription is initiated after RNA polymerase (RNAP) binds to promoter DNA, melts ~14 bp around the transcription start site and forms a single-stranded "transcription bubble" within a catalytically active...
5.
Le Reste L, Hohlbein J, Gryte K, Kapanidis A
Biophys J
. 2012 Jun;
102(11):2658-68.
PMID: 22713582
Dark quenchers are chromophores that primarily relax from the excited state to the ground state nonradiatively (i.e., are dark). As a result, they can serve as acceptors for Förster resonance...
6.
Uphoff S, Gryte K, Evans G, Kapanidis A
Chemphyschem
. 2011 Feb;
12(3):571-9.
PMID: 21280168
Switchable FRET is the combination of single-molecule Förster resonance energy transfer (smFRET) with photoswitching, the reversible activation and deactivation of fluorophores by light. By photoswitching, multiple donor-acceptor fluorophore pairs can...
7.
Cordes T, Santoso Y, Tomescu A, Gryte K, Hwang L, Camara B, et al.
Biochemistry
. 2010 Sep;
49(43):9171-80.
PMID: 20818825
Many biological processes, such as gene transcription and replication, involve opening and closing of short regions of double-stranded DNA (dsDNA). Few techniques, however, can study these processes in real time...
8.
Hohlbein J, Gryte K, Heilemann M, Kapanidis A
Phys Biol
. 2010 Aug;
7(3):031001.
PMID: 20686191
Single-molecule fluorescence microscopy is currently one of the most popular methods in the single-molecule toolbox. In this review, we discuss recent advances in fluorescence instrumentation and assays: these methods are...