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Keith Brocklehurst

Explore the profile of Keith Brocklehurst including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 21
Citations 140
Followers 0
Related Specialties
Biochemistry
Biomedical Engineering
Anatomy & Histology
Top 10 Co-Authors
Sheraz Gul
Marina Resmini
Emrys W Thomas
Syeed Hussain
Gerard Gallacher
Elizabeth L Ostler
Robert L Moritz
Sue-Hwa Lin
Albert J Courey
Guillaume Boucher
Published In
Biochem J
CSH Protoc
Biochemistry
Chem Commun (Camb)
J Immunol Methods
Affiliations
Soon will be listed here.
Recent Articles
1.
Cysteine proteases: mode of action and role in epidermal differentiation
Brocklehurst K, Philpott M
Cell Tissue Res . 2013 Jan; 351(2):237-44. PMID: 23344364
Desquamation or cell shedding in mammalian skin is known to involve serine proteases, aspartic proteases and glycosidases. In addition, evidence continues to accumulate that papain-like cysteine proteases and an inhibitor...
2.
DNA affinity chromatography
Brocklehurst K, Courey A, Gul S, Lin S, Moritz R
CSH Protoc . 2012 Apr; 2006(1). PMID: 22485607
No abstract available.
3.
Preparation of DNA affinity resin
Brocklehurst K, Courey A, Gul S, Lin S, Moritz R
CSH Protoc . 2012 Apr; 2006(1). PMID: 22485606
No abstract available.
4.
Directed Orientation of Antibodies during Preparation of Antibody Affinity Resin
Brocklehurst K, Courey A, Gul S, Lin S, Moritz R
CSH Protoc . 2012 Apr; 2006(1). PMID: 22485605
No abstract available.
5.
Protein ligand affinity chromatography
Brocklehurst K, Courey A, Gul S, Lin S, Moritz R
CSH Protoc . 2012 Apr; 2006(1). PMID: 22485604
No abstract available.
6.
Lectin-agarose affinity chromatography
Brocklehurst K, Courey A, Gul S, Lin S, Moritz R
CSH Protoc . 2012 Apr; 2006(1). PMID: 22485603
No abstract available.
7.
Identification of interactions involved in the generation of nucleophilic reactivity and of catalytic competence in the catalytic site Cys/His ion pair of papain
Hussain S, Khan A, Gul S, Resmini M, Verma C, Thomas E, et al.
Biochemistry . 2011 Nov; 50(49):10732-42. PMID: 22044167
Understanding the roles of noncovalent interactions within the enzyme molecule and between enzyme and substrate or inhibitor is an essential goal of the investigation of active center chemistry and catalytic...
8.
Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase
Seyedarabi A, To T, Ali S, Hussain S, Fries M, Madsen R, et al.
Biochemistry . 2009 Dec; 49(3):539-46. PMID: 20000851
Pectate lyases harness anti beta-elimination chemistry to cleave the alpha-1,4 linkage in the homogalacturonan region of plant cell wall pectin. We have studied the binding of five pectic oligosaccharides to...
9.
Generation of nucleophilic character in the Cys25/His159 ion pair of papain involves Trp177 but not Asp158
Gul S, Hussain S, Thomas M, Resmini M, Verma C, Thomas E, et al.
Biochemistry . 2008 Jan; 47(7):2025-35. PMID: 18225918
Studies on papain (EC 3.4.22.2), the most thoroughly investigated member of the cysteine proteinase superfamily, have contributed substantially to our understanding of the roles of noncovalent interactions in enzyme active...
10.
Molecular basis of the activity of the phytopathogen pectin methylesterase
Fries M, Ihrig J, Brocklehurst K, Shevchik V, Pickersgill R
EMBO J . 2007 Aug; 26(17):3879-87. PMID: 17717531
We provide a mechanism for the activity of pectin methylesterase (PME), the enzyme that catalyses the essential first step in bacterial invasion of plant tissues. The complexes formed in the...