Alpha 2.0
Login Register
» Authors » K M Ottemann

K M Ottemann

Explore the profile of K M Ottemann including associated specialties, affiliations and a list of published articles. Areas
Snapshot
Articles 8
Citations 388
Followers 0
Related Specialties
Microbiology
Molecular Biology
Science
Top 10 Co-Authors
D E Koshland Jr
J J Mekalanos
Y K Shin
J F Miller
V J DiRita
P J Schatz
T E Thorgeirsson
W Xiao
J Beckwith
K L Bieker
Published In
J Bacteriol
Mol Microbiol
Proc Natl Acad Sci U S A
EMBO J
Biochemistry
Affiliations
Soon will be listed here.
Recent Articles
1.
A piston model for transmembrane signaling of the aspartate receptor
Ottemann K, Xiao W, Shin Y, Koshland Jr D
Science . 1999 Sep; 285(5434):1751-4. PMID: 10481014
To characterize the mechanism by which receptors propagate conformational changes across membranes, nitroxide spin labels were attached at strategic positions in the bacterial aspartate receptor. By collecting the electron paramagnetic...
2.
Direct measurement of small ligand-induced conformational changes in the aspartate chemoreceptor using EPR
Ottemann K, Thorgeirsson T, Kolodziej A, Shin Y, Koshland Jr D
Biochemistry . 1998 Jun; 37(20):7062-9. PMID: 9585515
Ligand-binding-induced conformational changes in the Salmonella typhimurium aspartate receptor were studied using spin-labeling electron paramagnetic resonance. Cysteine residues, introduced by site-directed mutagenesis at several positions in the aspartate receptor periplasmic...
3.
Converting a transmembrane receptor to a soluble receptor: recognition domain to effector domain signaling after excision of the transmembrane domain
Ottemann K, Koshland Jr D
Proc Natl Acad Sci U S A . 1997 Oct; 94(21):11201-4. PMID: 9326586
The bacterial aspartate receptor was reconstructed to eliminate the transmembrane domain, thus connecting the recognition domain directly to the effector domain. The resulting soluble receptor folded correctly and was no...
4.
Roles for motility in bacterial-host interactions
Ottemann K, Miller J
Mol Microbiol . 1997 Jun; 24(6):1109-17. PMID: 9218761
The ability to move in a directed manner may confer distinct advantages upon host-adapted prokaryotes. Potential benefits of motility include increased efficiency of nutrient acquisition, avoidance of toxic substances, the...
5.
The ToxR protein of Vibrio cholerae forms homodimers and heterodimers
Ottemann K, Mekalanos J
J Bacteriol . 1996 Jan; 178(1):156-62. PMID: 8550410
The ToxR protein of Vibrio cholerae regulates the expression of several virulence factors that play important roles in the pathogenesis of cholera. Previous experiments with ToxR-alkaline phosphatase (ToxR-PhoA) fusion proteins...
6.
Analysis of Vibrio cholerae ToxR function by construction of novel fusion proteins
Ottemann K, Mekalanos J
Mol Microbiol . 1995 Feb; 15(4):719-31. PMID: 7783643
The ToxR protein is a transmembrane protein that regulates the expression of several virulence factors of Vibrio cholerae. Previous analysis of fusion proteins between ToxR and alkaline phosphatase (ToxR-PhoA) suggested...
7.
ToxR proteins with substitutions in residues conserved with OmpR fail to activate transcription from the cholera toxin promoter
Ottemann K, DiRita V, Mekalanos J
J Bacteriol . 1992 Nov; 174(21):6807-14. PMID: 1400230
The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly...
8.
One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery
Schatz P, Bieker K, Ottemann K, Silhavy T, Beckwith J
EMBO J . 1991 Jul; 10(7):1749-57. PMID: 2050112
The E. coli secE (prlG) gene codes for an integral cytoplasmic membrane protein which is part of the cell's secretory machinery. A deletion of nearly the entire gene renders the...