James M Piret
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Explore the profile of James M Piret including associated specialties, affiliations and a list of published articles.
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77
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3777
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Recent Articles
1.
Schulze H, Rangan S, Vardaki M, Blades M, Turner R, Piret J
Appl Spectrosc
. 2025 Feb;
:37028241311296.
PMID: 39894915
To better interpret the Raman spectra from mammalian cells, it is often desirable to reduce their complexity by decomposing them into the spectral contributions from individual macromolecules or types of...
2.
Kunitskaya A, Piret J
Cytotherapy
. 2025 Feb;
PMID: 39891634
Background: T-cell therapy advances have stimulated the development of bioprocesses to address the specialized needs of cell therapy manufacturing. During concentrated cell washing, the cells are frequently exposed to transiently...
3.
Moran M, Chen J, Piret J, Balcarcel R
Biotechnol Bioeng
. 2024 Apr;
121(10):3068-3075.
PMID: 38659198
Chinese hamster ovary (CHO) cells are widely used to manufacture biopharmaceuticals, most of all monoclonal antibodies (mAbs). Some CHO cell lines exhibit production instability, where the productivity of the cells...
4.
Iworima D, Baker R, Ellis C, Sherwood C, Zhan L, Rezania A, et al.
Stem Cell Res Ther
. 2024 Jan;
15(1):1.
PMID: 38167219
Background: Diabetes is a disease affecting over 500 million people globally due to insulin insufficiency or insensitivity. For individuals with type 1 diabetes, pancreatic islet transplantation can help regulate their...
5.
Iworima D, Baker R, Piret J, Kieffer T
Front Bioeng Biotechnol
. 2023 Dec;
11:1267007.
PMID: 38107616
Human pluripotent stem cells (hPSCs) provide many opportunities for application in regenerative medicine due to their ability to differentiate into cells from all three germ layers, proliferate indefinitely, and replace...
6.
Braam M, Zhao J, Liang S, Ida S, Kloostra N, Iworima D, et al.
Sci Rep
. 2023 Jun;
13(1):8877.
PMID: 37264038
The generation of functional β-cells from human pluripotent stem cells (hPSCs) for cell replacement therapy and disease modeling of diabetes is being investigated by many groups. We have developed a...
7.
Schulze H, Rangan S, Vardaki M, Blades M, Turner R, Piret J
Appl Spectrosc
. 2023 May;
77(8):957-969.
PMID: 37254554
Spectroscopic peak parameters are important since they provide information about the analyte under study. Besides obtaining these parameters, peak fitting also resolves overlapped peaks. Thus, the obtained parameters should permit...
8.
Rangan S, Wong R, Schulze H, Vardaki M, Blades M, Turner R, et al.
Analyst
. 2023 May;
148(12):2745-2757.
PMID: 37191142
Raman spectroscopy enables the label-free assessment of cellular composition. While live cell analysis is the most accurate approach for cellular Raman spectroscopy, the analysis of fixed cells has proved to...
9.
Gupta S, Shah B, Fung C, Chan P, Wakefield D, Kuhns S, et al.
Front Bioeng Biotechnol
. 2023 Mar;
11:1113994.
PMID: 36873370
Since 2015 more than 34 biosimilars have been approved by the FDA. This new era of biosimilar competition has stimulated renewed technology development focused on therapeutic protein or biologic manufacturing....
10.
Schulze H, Rangan S, Vardaki M, Blades M, Turner R, Piret J
Appl Spectrosc
. 2022 Oct;
77(8):835-847.
PMID: 36238996
Two-dimensional correlation spectroscopy (2D-COS) is a technique that permits the examination of synchronous and asynchronous changes present in hyperspectral data. It produces two-dimensional correlation coefficient maps that represent the mutually...