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J R Gautschi

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Articles 18
Citations 91
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1.
BAUMANN E, Gautschi J, Schindler R
Biochim Biophys Acta . 1979 Nov; 565(1):117-24. PMID: 508757
Cells from Chinese hamster ovary cell cultures were partially lysed with Brij-58 and incubated in a reaction mixture containing deoxyribonucleoside triphosphates and permitting semiconservative DNA replication. The reaction mixture was...
2.
Miller G, Schaer J, Gautschi J, Schindler R
Mol Cell Biochem . 1979 Sep; 27(1):7-15. PMID: 117296
In synchronous cultures of P-815 murine mastocytoma and of Chinese hamster ovary (CHO) cells, the relative contribution of exogenous thymidine to DNA synthesis was studied by comparing rates of (3H)thymidine...
3.
Reinhard P, Maillart P, Schluchter M, Gautschi J, Schindler R
Biochim Biophys Acta . 1979 Aug; 564(1):141-53. PMID: 534636
An assay for cellular factors stimulating DNA synthesis by partially lysed CHO cells is presented. The assay is based on the observation that in highly lysed cells, DNA synthesis, as...
4.
Hand R, Gautschi J
J Cell Biol . 1979 Aug; 82(2):485-93. PMID: 479312
We have used fiber autoradiography to examine the DNA product made in vitro in a lysed cell system. CHO cells were treated with 0.01% Brij-58 and the lysates were incubated...
5.
Gautschi J, Burkhalter M, BAUMANN E
Biochim Biophys Acta . 1978 Mar; 518(1):31-6. PMID: 629978
Relative utilization, of dThd, BrdUrd, and IdUrd as exogenous precursors of DNA in vivo was compared with utilization of the respective triphosphates in vitro. To measure relative utilization, exponentially growing...
6.
Ramseier H, Burkhalter M, Gautschi J
Exp Cell Res . 1977 Mar; 105(2):445-53. PMID: 139318
No abstract available.
7.
Gautschi J, Burkhalter M, Reinhard P
Biochim Biophys Acta . 1977 Feb; 474(4):512-23. PMID: 556954
DNA chain growing during semiconservative replication was studied using both in vitro systems described in the preceding paper (preceding paper, ref 1) 3H-Labeled, 4-S Okazaki fragments synthesized in vivo just...
8.
Reinhard P, Burkhalter M, Gautschi J
Biochim Biophys Acta . 1977 Feb; 474(4):500-11. PMID: 556953
To study DNA replication in vitro, mouse P-815 cells were either permeabilized by hypotonic treatment or gently lysed with the detergent Brij-58. In the presence of KCl, EGTA, creatine phosphokinase,...
9.
Hirsiger H, Gautschi J, Schindler R
Exp Cell Res . 1976 Jul; 100(2):356-64. PMID: 939260
No abstract available.
10.
Gautschi J, Clarkson J
Eur J Biochem . 1975 Jan; 50(2):403-12. PMID: 1168572
The length of newly synthesized DNA strands from mouse P-815 cells was analyzed after denaturation both by electrophoresis and by sedimentation in alkaline sucrose gradients. [3-H]-Thymidine pulses of 2-8 min...