J B Crechet
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Explore the profile of J B Crechet including associated specialties, affiliations and a list of published articles.
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19
Citations
144
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Recent Articles
1.
Crechet J, Jacquet E, Bernardi A, Parmeggiani A
J Biol Chem
. 2000 Apr;
275(23):17754-61.
PMID: 10747953
Ras proteins from Saccharomyces cerevisiae differ from mammalian Ha-Ras in their extended C-terminal hypervariable region. We have analyzed the function of this region and the effect of its farnesylation with...
2.
Distal switch II region of Ras2p is required for interaction with guanine nucleotide exchange factor
Crechet J, Bernardi A, Parmeggiani A
J Biol Chem
. 1996 Jul;
271(29):17234-40.
PMID: 8663268
The interaction of Saccharomyces cerevisiae Ras2p with the catalytic domain of the GDP/GTP exchange factors (GEFs) mouse CDC25(Mm), yeast Cdc25p, and Sdc25p was analyzed by introducing the substitution R80D/N81D into...
3.
Poullet P, Crechet J, Bernardi A, Parmeggiani A
Eur J Biochem
. 1995 Jan;
227(1-2):537-44.
PMID: 7851434
The catalytic domain of the Saccharomyces cerevisiae SDC25 gene product, including the last 550 C-terminal residues (Sdc25p-C), was produced as an Escherichia coli recombinant protein fused with glutathione S-transferase. The...
4.
Crechet J, Poullet P, Bernardi A, Fasano O, Parmeggiani A
J Biol Chem
. 1993 Jul;
268(20):14836-41.
PMID: 8325863
The SDC25 C-domain, the product encoded by the 3'-terminal part of the Saccharomyces cerevisiae SDC25 gene, acts as a GDP dissociation stimulator on RAS proteins (Créchet, J.B., Poullet, P., Mistou,...
5.
Verrotti A, Crechet J, Di Blasi F, Seidita G, Mirisola M, Kavounis C, et al.
EMBO J
. 1992 Aug;
11(8):2855-62.
PMID: 1639059
We have previously shown that a conserved glycine at position 82 of the yeast RAS2 protein is involved in the conversion of RAS proteins from the GDP- to the GTP-bound...
6.
Kavounis C, Verrotti A, De Vendittis E, Bozopoulos A, Di Blasi F, Zahn R, et al.
FEBS Lett
. 1991 Apr;
281(1-2):235-9.
PMID: 1901802
Ras proteins bind either GDP or GTP with high affinity. However, only the GTP-bound form of the yeast Ras2 protein is able to stimulate adenylyl cyclase. To identify amino acid...
7.
Crechet J, Poullet P, Mistou M, Parmeggiani A, Camonis J, Boy-Marcotte E, et al.
Science
. 1990 May;
248(4957):866-8.
PMID: 2188363
In Saccharomyces cerevisiae, the product of the CDC25 gene controls the RAS-mediated production of adenosine 3',5'-monophosphate (cAMP). In vivo the carboxyl-terminal third of the CDC25 gene product is sufficient for...
8.
Crechet J, Poullet P, Camonis J, Jacquet M, Parmeggiani A
J Biol Chem
. 1990 Jan;
265(3):1563-8.
PMID: 2104846
The properties of RAS2Gly19----Val and RAS2Thr152----Ile, two mutants suppressing the CDC25 requirement for the activation of adenylate cyclase in Saccharomyces cerevisiae, were compared with the properties of wild-type RAS2. We...
9.
Fasano O, Crechet J, De Vendittis E, Zahn R, Feger G, Vitelli A, et al.
EMBO J
. 1988 Nov;
7(11):3375-83.
PMID: 3145192
Saccharomyces cerevisiae strains with a disrupted RAS1 gene and with an intact RAS2 gene (ras1- RAS2 strains) grew well on both fermentable and nonfermentable carbon sources. By constructing isogenic mutants...
10.
Crechet J, Parmeggiani A
Eur J Biochem
. 1986 Dec;
161(3):647-53.
PMID: 3641717
The properties of EF-1 alpha from calf brain have been investigated and compared with those of EF-Tu. EF-1 alpha binds GDP and GTP in a 1:1 stoichiometry, showing the same...