H Perry-OKeefe
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Explore the profile of H Perry-OKeefe including associated specialties, affiliations and a list of published articles.
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Articles
13
Citations
184
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Recent Articles
1.
Perry-OKeefe H, Kissinger C
Curr Protoc Mol Biol
. 2008 Feb;
Chapter 3:Unit3.19.
PMID: 18265227
With recent advances in techniques for detecting chemiluminescent substrates, hybridization with nonisotopic rather than radiolabeled probes is becoming more common. In the Basic Protocol, nylon membranes carrying transferred nucleic acids...
2.
Boyle A, Perry-OKeefe H
Curr Protoc Mol Biol
. 2008 Feb;
Chapter 3:Unit3.18.
PMID: 18265226
Although a number of different nonisotopic labels have been described in the literature, biotin and digoxigenin are used most frequently and are commercially available. Either label can be easily incorporated...
3.
Radwanska M, Magez S, Perry-OKeefe H, Stender H, Coull J, Sternberg J, et al.
J Clin Microbiol
. 2002 Nov;
40(11):4295-7.
PMID: 12409413
We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes...
4.
Perry-OKeefe H, Rigby S, Oliveira K, Sorensen D, Stender H, Coull J
J Microbiol Methods
. 2001 Nov;
47(3):281-92.
PMID: 11714518
A standardized fluorescent in situ hybridization (FISH) method using Peptide Nucleic Acid (PNA) probes for analysis of gram-negative and gram-positive bacteria, as well as yeast, has been developed. Fluorescently labeled...
5.
Perry-OKeefe H, Stender H, Broomer A, Oliveira K, Coull J
J Appl Microbiol
. 2001 Feb;
90(2):180-9.
PMID: 11168720
Aims: A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. Methods And Results: The method is based on...
6.
Stender H, Kurtzman C, Sorensen D, Broomer A, Oliveira K, Perry-OKeefe H, et al.
Appl Environ Microbiol
. 2001 Feb;
67(2):938-41.
PMID: 11157265
A new fluorescence in situ hybridization method using peptide nucleic acid (PNA) probes for identification of Brettanomyces is described. The test is based on fluorescein-labeled PNA probes targeting a species-specific...
7.
Stender H, Broomer A, Oliveira K, Perry-OKeefe H, Sage A, Coull J
Appl Environ Microbiol
. 2001 Jan;
67(1):142-7.
PMID: 11133438
A new chemiluminescent in situ hybridization (CISH) method provides simultaneous detection, identification, and enumeration of culturable Escherichia coli cells in 100 ml of municipal water within one working day. Following...
8.
Stender H, Broomer A, Oliveira K, Perry-OKeefe H, Sage A, Young B, et al.
J Microbiol Methods
. 2000 Oct;
42(3):245-53.
PMID: 11044568
A new chemiluminescent in situ hybridization (CISH) method that provides simultaneous detection, identification, and enumeration of Pseudomonas aeruginosa in bottled water within 1 working day has been developed. Individual micro-colonies...
9.
Perry-OKeefe H, Yao X, Coull J, Fuchs M, Egholm M
Proc Natl Acad Sci U S A
. 1996 Dec;
93(25):14670-5.
PMID: 8962112
We have found that it is possible to use labeled peptide nucleic acid (PNA)-oligomers as probes in pre-gel hybridization experiments, as an alternative for Southern hybridization. In this technique, the...
10.
Sears L, Moran L, Kissinger C, Creasey T, Perry-OKeefe H, Roskey M, et al.
Biotechniques
. 1992 Oct;
13(4):626-33.
PMID: 1476733
CircumVent thermal cycle and standard DNA sequencing protocols utilizing the cloned and highly thermostable VentR (exo-) DNA polymerase are described. The thermal cycle sequencing procedures are advantageous because they allow...