Ghanshyam D Heda
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Explore the profile of Ghanshyam D Heda including associated specialties, affiliations and a list of published articles.
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13
Citations
156
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Recent Articles
1.
Heda G
Biotechniques
. 2020 Nov;
70(1):54-57.
PMID: 33222512
The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which...
2.
Heda G, Shrestha L, Thapa S, Ghimire S, Raut D
Biotechniques
. 2020 Apr;
68(6):318-324.
PMID: 32283940
Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting....
3.
Heda G, Omotola O, Heda R, Avery J
J Biomol Tech
. 2016 Sep;
27(3):113-8.
PMID: 27582639
SDS-PAGE and Western blotting are 2 of the most commonly used biochemical methods for protein analysis. Proteins are electrophoretically separated based on their MWs by SDS-PAGE and then electrotransferred to...
4.
Heda G, Kunwar U, Heda R
Anal Biochem
. 2013 Oct;
445:67-72.
PMID: 24135655
In this article, we present a modified and improved protein assay that was previously described as "amidoschwarz assay" by Schaffner and Weissmann. Our improved protein assay is user-friendly and 30-40...
5.
Heda G, Roberts L
J Am Podiatr Med Assoc
. 2008 Sep;
98(5):345-52.
PMID: 18820035
Background: Plasminogen activators (PAs) and their regulatory counterparts, PA inhibitors (PAIs), play a role in normal differentiation processes and various pathophysiologic conditions of the epidermis. Normal desquamation of corneocytes from...
6.
Norez C, Heda G, Jensen T, Kogan I, Hughes L, Auzanneau C, et al.
J Cyst Fibros
. 2004 Oct;
3 Suppl 2:119-21.
PMID: 15463942
Flux studies using either radioisotopes or ion-selective electrodes are a convenient method to assay the function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel. Here, we described three...
7.
Roxo-Rosa M, Davezac N, Bensalem N, Majumder M, Heda G, Simas A, et al.
J Cyst Fibros
. 2004 Oct;
3 Suppl 2:85-9.
PMID: 15463934
Numerous factors, other than mutations in the CFTR gene, affect the phenotypic variability of cystic fibrosis (CF). With a two-dimensional electrophoresis (2-DE) analysis of total protein expression profiles (proteomics) of...
8.
Farinha C, Penque D, Roxo-Rosa M, Lukacs G, Dormer R, McPherson M, et al.
J Cyst Fibros
. 2004 Oct;
3 Suppl 2:73-7.
PMID: 15463932
Detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein is usually a difficult task to accomplish due to the low levels of expression and high turnover that this membrane protein...
9.
Mendes F, Farinha C, Roxo-Rosa M, Fanen P, Edelman A, Dormer R, et al.
J Cyst Fibros
. 2004 Oct;
3 Suppl 2:69-72.
PMID: 15463931
For most expression studies focusing on the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein, sensitive and specific antibodies (Abs) are critically needed. Several Abs have been produced commercially or by...
10.
Trezise A, Farinha C, Heda G, Harris A, Amaral M, Mouchel N
J Cyst Fibros
. 2004 Oct;
3 Suppl 2:25-8.
PMID: 15463920
Cystic fibrosis transmembrane conductance regulator gene (CFTR) shows a complex mechanism of tissue-specific and temporal regulation. CFTR mRNA detection and measurement are extremely difficult because of the low to very...