David Bergenholm
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Explore the profile of David Bergenholm including associated specialties, affiliations and a list of published articles.
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12
Citations
155
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Recent Articles
1.
Bergenholm D, Dabirian Y, Ferreira R, Siewers V, David F, Nielsen J
Synth Biol (Oxf)
. 2021 Oct;
6(1):ysab014.
PMID: 34712839
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has become a standard tool in many genome engineering endeavors. The endonuclease-deficient version of Cas9 (dCas9) is also a powerful programmable...
2.
Borlin C, Bergenholm D, Holland P, Nielsen J
Biol Methods Protoc
. 2020 May;
4(1):bpz011.
PMID: 32395628
The decrease of sequencing cost in the recent years has made genome-wide studies of transcription factor (TF) binding through chromatin immunoprecipitation methods like ChIP-seq and chromatin immunoprecipitation with lambda exonuclease...
3.
Holland P, Bergenholm D, Borlin C, Liu G, Nielsen J
Nucleic Acids Res
. 2019 Apr;
47(10):4986-5000.
PMID: 30976803
Transcription factors (TF) are central to transcriptional regulation, but they are often studied in relative isolation and without close control of the metabolic state of the cell. Here, we describe...
4.
Bergenholm D, Liu G, Hansson D, Nielsen J
Biotechnol Bioeng
. 2019 Jan;
116(5):1029-1038.
PMID: 30659597
To achieve large-scale, high-throughput experiments for systems biology research of microorganisms, reliable data from robust cultivation systems are needed. Chemostats are such systems, ensuring reproducibility and quality by providing a...
5.
Saccharomyces cerevisiae displays a stable transcription start site landscape in multiple conditions
Borlin C, Cvetesic N, Holland P, Bergenholm D, Siewers V, Lenhard B, et al.
FEMS Yeast Res
. 2018 Dec;
19(2).
PMID: 30590648
One of the fundamental processes that determine cellular fate is regulation of gene transcription. Understanding these regulatory processes is therefore essential for understanding cellular responses to changes in environmental conditions....
6.
Ouyang L, Holland P, Lu H, Bergenholm D, Nielsen J
FEMS Yeast Res
. 2018 Aug;
18(8).
PMID: 30107458
The Saccharomyces cerevisiae transcription factor (TF) Stb5 is known to be involved in regulating NADPH generation. We explored its role by combining DNA binding studies with transcriptome analysis at four...
7.
Bergenholm D, Liu G, Holland P, Nielsen J
mSystems
. 2018 Aug;
3(4).
PMID: 30073202
To build transcription regulatory networks, transcription factor binding must be analyzed in cells grown under different conditions because their responses and targets differ depending on environmental conditions. We performed whole-genome...
8.
Wei Y, Bergenholm D, Gossing M, Siewers V, Nielsen J
Microb Cell Fact
. 2018 Jan;
17(1):11.
PMID: 29370801
Background: Cocoa butter (CB) extracted from cocoa beans (Theobroma cacao) is the main raw material for chocolate production, but CB supply is insufficient due to the increased chocolate demand and...
9.
Bergenholm D, Gossing M, Wei Y, Siewers V, Nielsen J
Biotechnol Bioeng
. 2018 Jan;
115(4):932-942.
PMID: 29313898
Chain length and degree of saturation plays an important role for the characteristics of various products derived from fatty acids, such as fuels, cosmetics, and food additives. The seeds of...
10.
Wei Y, Gossing M, Bergenholm D, Siewers V, Nielsen J
AMB Express
. 2017 Feb;
7(1):34.
PMID: 28168573
Cocoa butter (CB) extracted from cocoa beans mainly consists of three different kinds of triacylglycerols (TAGs), 1,3-dipalmitoyl-2-oleoyl-glycerol (POP, C16:0-C18:1-C16:0), 1-palmitoyl-3-stearoyl-2-oleoyl-glycerol (POS, C16:0-C18:1-C18:0) and 1,3-distearoyl-2-oleoyl-glycerol (SOS, C18:0-C18:1-C18:0), but CB supply is...