David A OBrochta
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Explore the profile of David A OBrochta including associated specialties, affiliations and a list of published articles.
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36
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1355
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Recent Articles
11.
Reid W, OBrochta D
Curr Opin Insect Sci
. 2016 Jul;
13:43-54.
PMID: 27436552
Insect genome editing was first reported 1991 in Drosophila melanogaster but the technology used was not portable to other species. Not until the recent development of facile, engineered DNA endonuclease...
12.
Xu H, OBrochta D
Proc Biol Sci
. 2015 Jun;
282(1810).
PMID: 26108630
Genetic technologies based on transposon-mediated transgenesis along with several recently developed genome-editing technologies have become the preferred methods of choice for genetically manipulating many organisms. The silkworm, Bombyx mori, is...
13.
Duan J, Xu H, Guo H, OBrochta D, Wang F, Ma S, et al.
PLoS One
. 2013 Nov;
8(11):e79703.
PMID: 24244545
Sex-determination mechanisms differ among organisms. The primary mechanism is diverse, whereas the terminal regulator is relatively-conserved. We analyzed the transcripts of the Bombyx mori doublesex gene (Bmdsx), and reported novel...
14.
Palavesam A, Esnault C, OBrochta D
PLoS One
. 2013 Jul;
8(7):e68454.
PMID: 23861905
The piggyBac transposon, originating in the genome of the Lepidoptera Trichoplusia ni, has a broad host range, making it useful for the development of a number of transposon-based functional genomic...
15.
OBrochta D, Pilitt K, Harrell 2nd R, Aluvihare C, Alford R
G3 (Bethesda)
. 2012 Nov;
2(11):1305-15.
PMID: 23173082
Transposon-based forward and reverse genetic technologies will contribute greatly to ongoing efforts to study mosquito functional genomics. A piggyBac transposon-based enhancer-trap system was developed that functions efficiently in the human...
16.
OBrochta D, Alford R, Pilitt K, Aluvihare C, Harrell 2nd R
Proc Natl Acad Sci U S A
. 2011 Sep;
108(39):16339-44.
PMID: 21930941
Technical advances in mosquito biology are enabling the development of new approaches to vector control. Absent are powerful forward-genetics technologies, such as enhancer and gene traps, that permit determination of...
17.
Kim Y, Hice R, OBrochta D, Atkinson P
Genetica
. 2011 Aug;
139(8):985-97.
PMID: 21805320
We have conducted a structure and functional analysis of the hobo transposable element of Drosophila melanogaster. A minimum of 141 bp of the left (L) end and 65 bp of...
18.
Kahlon A, Hice R, OBrochta D, Atkinson P
Mob DNA
. 2011 Jun;
2(1):9.
PMID: 21689391
Background: Determining the mechanisms by which transposable elements move within a genome increases our understanding of how they can shape genome evolution. Class 2 transposable elements transpose via a 'cut-and-paste'...
19.
Arensburger P, Hice R, Zhou L, Smith R, Tom A, Wright J, et al.
Genetics
. 2011 Mar;
188(1):45-57.
PMID: 21368277
Transposons are found in virtually all organisms and play fundamental roles in genome evolution. They can also acquire new functions in the host organism and some have been developed as...
20.
Esnault C, Palavesam A, Pilitt K, OBrochta D
Genetics
. 2010 Oct;
187(1):319-31.
PMID: 20944016
Identifying factors influencing transposable element activity is essential for understanding how these elements impact genomes and their evolution as well as for fully exploiting them as functional genomics tools and...