Amanda Haupt
Overview
Explore the profile of Amanda Haupt including associated specialties, affiliations and a list of published articles.
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Articles
7
Citations
291
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0
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Recent Articles
1.
Gregor B, Coston M, Adams E, Arakaki J, Borensztejn A, Do T, et al.
Nat Protoc
. 2023 Dec;
19(2):565-594.
PMID: 38087082
To produce abundant cell culture samples to generate large, standardized image datasets of human induced pluripotent stem (hiPS) cells, we developed an automated workflow on a Hamilton STAR liquid handler...
2.
Viana M, Chen J, Knijnenburg T, Vasan R, Yan C, Arakaki J, et al.
Nature
. 2023 Jan;
613(7943):345-354.
PMID: 36599983
Understanding how a subset of expressed genes dictates cellular phenotype is a considerable challenge owing to the large numbers of molecules involved, their combinatorics and the plethora of cellular behaviours...
3.
Mills C, Riching A, Keller A, Stombaugh J, Haupt A, Maksimova E, et al.
CRISPR J
. 2022 Oct;
5(6):769-786.
PMID: 36257604
While CRISPR interference (CRISPRi) systems have been widely implemented in pooled lentiviral screening, there has been limited use with synthetic guide RNAs for the complex phenotypic readouts enabled by experiments...
4.
Roberts B, Hendershott M, Arakaki J, Gerbin K, Malik H, Nelson A, et al.
Stem Cell Reports
. 2019 Apr;
12(5):1145-1158.
PMID: 30956114
We describe a multistep method for endogenous tagging of transcriptionally silent genes in human induced pluripotent stem cells (hiPSCs). A monomeric EGFP (mEGFP) fusion tag and a constitutively expressed mCherry...
5.
Haupt A, Grancharova T, Arakaki J, Fuqua M, Roberts B, Gunawardane R
J Vis Exp
. 2018 Sep;
(138).
PMID: 30199041
A protocol is presented for generating human induced pluripotent stem cells (hiPSCs) that express endogenous proteins fused to in-frame N- or C-terminal fluorescent tags. The prokaryotic CRISPR/Cas9 system (clustered regularly...
6.
Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua M, et al.
Mol Biol Cell
. 2017 Aug;
28(21):2854-2874.
PMID: 28814507
We present a CRISPR/Cas9 genome-editing strategy to systematically tag endogenous proteins with fluorescent tags in human induced pluripotent stem cells (hiPSC). To date, we have generated multiple hiPSC lines with...
7.
Anderson E, Haupt A, Schiel J, Chou E, Machado H, Strezoska Z, et al.
J Biotechnol
. 2015 Jul;
211:56-65.
PMID: 26189696
The discovery that the bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) acquired immune system can be utilized to create double-strand breaks (DSBs) in eukaryotic genomes has...