Alexander A Sousa
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Explore the profile of Alexander A Sousa including associated specialties, affiliations and a list of published articles.
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12
Citations
3158
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Recent Articles
1.
Sousa A, Hemez C, Lei L, Traore S, Kulhankova K, Newby G, et al.
Nat Biomed Eng
. 2024 Jul;
9(1):7-21.
PMID: 38987629
Prime editing (PE) enables precise and versatile genome editing without requiring double-stranded DNA breaks. Here we describe the systematic optimization of PE systems to efficiently correct human cystic fibrosis (CF)...
2.
Chen L, Hong M, Luan C, Gao H, Ru G, Guo X, et al.
Nat Biotechnol
. 2024 Apr;
42(6):987.
PMID: 38658732
No abstract available.
3.
Chen L, Hong M, Luan C, Gao H, Ru G, Guo X, et al.
Nat Biotechnol
. 2023 Jun;
42(4):638-650.
PMID: 37322276
Base editors have substantial promise in basic research and as therapeutic agents for the correction of pathogenic mutations. The development of adenine transversion editors has posed a particular challenge. Here...
4.
Everette K, Newby G, Levine R, Mayberry K, Jang Y, Mayuranathan T, et al.
Nat Biomed Eng
. 2023 Apr;
7(5):616-628.
PMID: 37069266
Sickle-cell disease (SCD) is caused by an A·T-to-T·A transversion mutation in the β-globin gene (HBB). Here we show that prime editing can correct the SCD allele (HBB) to wild type...
5.
Doman J, Sousa A, Randolph P, Chen P, Liu D
Nat Protoc
. 2022 Aug;
17(11):2431-2468.
PMID: 35941224
Prime editing (PE) is a precision gene editing technology that enables the programmable installation of substitutions, insertions and deletions in cells and animals without requiring double-strand DNA breaks (DSBs). The...
6.
Kleinstiver B, Sousa A, Walton R, Tak Y, Hsu J, Clement K, et al.
Nat Biotechnol
. 2020 Jun;
38(7):901.
PMID: 32541959
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
7.
Anzalone A, Randolph P, Davis J, Sousa A, Koblan L, Levy J, et al.
Nature
. 2019 Oct;
576(7785):149-157.
PMID: 31634902
Most genetic variants that contribute to disease are challenging to correct efficiently and without excess byproducts. Here we describe prime editing, a versatile and precise genome editing method that directly...
8.
Hanlon K, Kleinstiver B, Garcia S, Zaborowski M, Volak A, Spirig S, et al.
Nat Commun
. 2019 Oct;
10(1):4439.
PMID: 31570731
Adeno-associated virus (AAV) vectors have shown promising results in preclinical models, but the genomic consequences of transduction with AAV vectors encoding CRISPR-Cas nucleases is still being examined. In this study,...
9.
Kleinstiver B, Sousa A, Walton R, Tak Y, Hsu J, Clement K, et al.
Nat Biotechnol
. 2019 Feb;
37(3):276-282.
PMID: 30742127
Broad use of CRISPR-Cas12a (formerly Cpf1) nucleases has been hindered by the requirement for an extended TTTV protospacer adjacent motif (PAM). To address this limitation, we engineered an enhanced Acidaminococcus...
10.
Marino N, Zhang J, Borges A, Sousa A, Leon L, Rauch B, et al.
Science
. 2018 Sep;
362(6411):240-242.
PMID: 30190308
Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit the immune function of CRISPR-Cas. To...