Alex Y Liu
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Explore the profile of Alex Y Liu including associated specialties, affiliations and a list of published articles.
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12
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43
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Recent Articles
1.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2019 Dec;
2019(12).
PMID: 31792145
Several different methodologies for mutagenesis have been developed to introduce mutations at predetermined sites or regions within mammalian genes. These methods of in vitro mutagenesis have had a transforming effect...
2.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2019 Dec;
2019(12).
PMID: 31792139
This protocol, suitable for both general and close-proximity mutagenesis, includes a simple and rapid procedure that combines polymerase chain reaction (PCR), DpnI digestion, and overlap extension. The key point of...
3.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2019 Jun;
2019(6).
PMID: 31160386
The megaprimer method is a simple and versatile approach that can be adopted to create a single mutation in a specific target region as well as to create site-specific insertions,...
4.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2019 Jun;
2019(6).
PMID: 31160385
In vitro oligonucleotide and polymerase chain reaction (PCR)-based mutagenesis is generally used for altering the nucleotide sequence of genes to study their functional importance and the products they encode. A...
5.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2019 Jan;
2019(1).
PMID: 30602556
Saturation mutagenesis by cassette insertion introduces a library of site-specific changes into a specific DNA sequence within a target gene and is especially useful for analyzing the effect of specific...
6.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2019 Jan;
2019(1).
PMID: 30602555
In this method, two oligonucleotide primers are hybridized to the same strand of a denatured double-stranded recombinant plasmid. One primer (the mutagenic primer) introduces the desired mutation into the target...
7.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2018 Aug;
2018(8).
PMID: 30068589
Many protocols exist to perform site-directed mutagenesis, and here we present one of the more commonly used ones-site-directed mutagenesis by altered β-lactamase selection. β-Lactamase is an enzyme that cleaves ampicillin,...
8.
Creating Insertions or Deletions Using Overlap Extension Polymerase Chain Reaction (PCR) Mutagenesis
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2018 Aug;
2018(8).
PMID: 30068588
Overlap extension polymerase chain reaction (PCR) mutagenesis can be used for the generation of a specific point mutation, insertion, or deletion within a particular DNA sequence of interest. It requires...
9.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2018 Mar;
2018(3).
PMID: 29496819
In this protocol, two oligonucleotides are used to prime DNA synthesis by a high-fidelity polymerase on a denatured plasmid template. The two oligonucleotides both contain the desired mutation and have...
10.
Forloni M, Liu A, Wajapeyee N
Cold Spring Harb Protoc
. 2018 Mar;
2018(3).
PMID: 29496818
"Random mutagenesis" is a technique that allows researchers to develop large libraries of variants of a particular DNA sequence. Once developed, these libraries can then be used for several purposes,...