The Signal Transduction Protein GlnK is Required for NifL-dependent Nitrogen Control of Nif Gene Expression in Klebsiella Pneumoniae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1999 Feb 11

PMID 9973341

Citations 33

Authors

R Jack

M de Zamaroczy

M Merrick

Affiliations

Soon will be listed here.

Abstract

In Klebsiella pneumoniae, transcription of the nitrogen fixation (nif) genes is regulated in response to molecular oxygen or availability of fixed nitrogen by the coordinated activities of the nifA and nifL gene products. NifA is a nif-specific transcriptional activator, the activity of which is inhibited by interaction with NifL. Nitrogen control of NifL occurs at two levels: transcription of the nifLA operon is regulated by the global ntr system, and the inhibitory activity of NifL is controlled in response to fixed nitrogen by an unknown factor. K. pneumoniae synthesizes two PII-like signal transduction proteins, GlnB, which we have previously shown not to be involved in the response of NifL to fixed nitrogen, and the recently identified protein GlnK. We have now cloned the K. pneumoniae glnK gene, studied its expression, and shown that a null mutation in glnK prevents NifL from responding to the absence of fixed nitrogen, i.e., from relieving the inhibition of NifA activity. Hence, GlnK appears to be involved, directly or indirectly, in NifL-dependent regulation of nif gene expression in K. pneumoniae. Comparison of the GlnB and GlnK amino acid sequences from six species of proteobacteria identifies five residues (residues 3, 5, 52, 54, and 64) which serve to distinguish the GlnB and GlnK proteins.

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References

Merrick M . Nitrogen control of the nif regulon in Klebsiella pneumoniae: involvement of the ntrA gene and analogies between ntrC and nifA. EMBO J. 1983; 2(1):39-44. PMC: 555083. DOI: 10.1002/j.1460-2075.1983.tb01377.x. View

Liang Y, de Zamaroczy M, Arsene F, PAQUELIN A, Elmerich C . Regulation of nitrogen fixation in Azospirillum brasilense Sp7: involvement of nifA, glnA and glnB gene products. FEMS Microbiol Lett. 1992; 100(1-3):113-9. DOI: 10.1111/j.1574-6968.1992.tb14028.x. View

Carr P, Cheah E, Suffolk P, Vasudevan S, Dixon N, Ollis D . X-ray structure of the signal transduction protein from Escherichia coli at 1.9 A. Acta Crystallogr D Biol Crystallogr. 1996; 52(Pt 1):93-104. DOI: 10.1107/S0907444995007293. View

Contreras A, Drummond M, Bali A, Blanco G, Garcia E, Bush G . The product of the nitrogen fixation regulatory gene nfrX of Azotobacter vinelandii is functionally and structurally homologous to the uridylyltransferase encoded by glnD in enteric bacteria. J Bacteriol. 1991; 173(24):7741-9. PMC: 212563. DOI: 10.1128/jb.173.24.7741-7749.1991. View

Coppard J, Merrick M . Cassette mutagenesis implicates a helix-turn-helix motif in promoter recognition by the novel RNA polymerase sigma factor sigma 54. Mol Microbiol. 1991; 5(6):1309-17. DOI: 10.1111/j.1365-2958.1991.tb00777.x. View

Contreras A, Drummond M . Cys184 and Cys187 of NifL protein of Klebsiella pneumoniae are not absolutely required for inhibition of NifA activity. Gene. 1991; 103(1):83-6. DOI: 10.1016/0378-1119(91)90395-r. View

Holtel A, Merrick M . The Klebsiella pneumoniae PII protein (glnB gene product) is not absolutely required for nitrogen regulation and is not involved in NifL-mediated nif gene regulation. Mol Gen Genet. 1989; 217(2-3):474-80. DOI: 10.1007/BF02464920. View

Sawers G, Bock A . Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression. J Bacteriol. 1989; 171(5):2485-98. PMC: 209925. DOI: 10.1128/jb.171.5.2485-2498.1989. View

Kustu S, Santero E, Keener J, Popham D, Weiss D . Expression of sigma 54 (ntrA)-dependent genes is probably united by a common mechanism. Microbiol Rev. 1989; 53(3):367-76. PMC: 372741. DOI: 10.1128/mr.53.3.367-376.1989. View

10.

Reitzer L, MAGASANIK B . Expression of glnA in Escherichia coli is regulated at tandem promoters. Proc Natl Acad Sci U S A. 1985; 82(7):1979-83. PMC: 397465. DOI: 10.1073/pnas.82.7.1979. View

11.

Bueno R, Pahel G, MAGASANIK B . Role of glnB and glnD gene products in regulation of the glnALG operon of Escherichia coli. J Bacteriol. 1985; 164(2):816-22. PMC: 214324. DOI: 10.1128/jb.164.2.816-822.1985. View

12.

Toukdarian A, Kennedy C . Regulation of nitrogen metabolism in Azotobacter vinelandii: isolation of ntr and glnA genes and construction of ntr mutants. EMBO J. 1986; 5(2):399-407. PMC: 1166745. DOI: 10.1002/j.1460-2075.1986.tb04225.x. View

13.

Riccio A, Guida M, Defez R, Lamberti A, Iaccarino M, Arnold W . Tight linkage of glnA and a putative regulatory gene in Rhizobium leguminosarum. Nucleic Acids Res. 1987; 15(5):1951-64. PMC: 340610. DOI: 10.1093/nar/15.5.1951. View

14.

Son H, Rhee S . Cascade control of Escherichia coli glutamine synthetase. Purification and properties of PII protein and nucleotide sequence of its structural gene. J Biol Chem. 1987; 262(18):8690-5. View

15.

Holtel A, Merrick M . Identification of the Klebsiella pneumoniae glnB gene: nucleotide sequence of wild-type and mutant alleles. Mol Gen Genet. 1988; 215(1):134-8. DOI: 10.1007/BF00331314. View

16.

Drummond M, Whitty P, Wootton J . Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins. EMBO J. 1986; 5(2):441-7. PMC: 1166750. DOI: 10.1002/j.1460-2075.1986.tb04230.x. View

17.

Santero E, Toukdarian A, Humphrey R, Kennedy C . Identification and characterization of two nitrogen fixation regulatory regions, nifA and nfrX, in Azotobacter vinelandii and Azotobacter chroococcum. Mol Microbiol. 1988; 2(3):303-14. DOI: 10.1111/j.1365-2958.1988.tb00033.x. View

18.

Dixon R, Kennedy C, Kondorosi A, Krishnapillai V, Merrick M . Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation. Mol Gen Genet. 1977; 157(2):189-98. DOI: 10.1007/BF00267397. View

19.

Minton N . Improved plasmid vectors for the isolation of translational lac gene fusions. Gene. 1984; 31(1-3):269-73. DOI: 10.1016/0378-1119(84)90220-8. View

20.

Backman K, Chen Y, MAGASANIK B . Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1981; 78(6):3743-7. PMC: 319648. DOI: 10.1073/pnas.78.6.3743. View