Alpha 2.0
Login Register
» Articles » PMID: 994751

High Pressure Reverse Phase Liquid Chromatography of Fatty Acid P-bromophenacyl Esters

Overview
Journal Lipids
Specialty Biochemistry
Date 1976 Nov 1
PMID 994751
Citations 8
Authors
P T Pei
W C Kossa
S Ramachandran
R S HENLY
Affiliations
Soon will be listed here.
Abstract

High pressure reverse phase liquid chromatography has been employed to rapidly separate saturated and unsaturated fatty acids as the corresponding p-bromophenacyl esters. Through the use of a highly efficient C18 reverse phase column packing, it has also been possible to distinguish among geometrical and positional isomers of the unsaturated acids. The use of ultroviolet-sensitive esters has permitted the detection of low (nanogram range) concentrations of fatty acids. The time required for analysis has been further reduced by employing a novel and rapid method for the preparation of the esters.

Citing Articles

High performance liquid chromatography and glass capillary gas chromatography of geometric and positional isomers of long chain monounsaturated fatty acids.

Svensson L, Sisfontes L, Nyborg G, Blomstrand R Lipids. 2016; 17(1):50-9.

PMID: 27519308 DOI: 10.1007/BF02535122.

Mycolic acid analysis by high-performance liquid chromatography for identification of Mycobacterium species.

Butler W, Guthertz L Clin Microbiol Rev. 2001; 14(4):704-26, table of contents.

PMID: 11585782 PMC: 88994. DOI: 10.1128/CMR.14.4.704-726.2001.

Mevinolinic acid biosynthesis by Aspergillus terreus and its relationship to fatty acid biosynthesis.

GREENSPAN M, Yudkovitz J J Bacteriol. 1985; 162(2):704-7.

PMID: 3988710 PMC: 218907. DOI: 10.1128/jb.162.2.704-707.1985.

Determination of the amounts of free arachidonic acid in resident and activated rabbit alveolar macrophages by fluorometric high performance liquid chromatography.

Nakagawa Y, WAKU K Lipids. 1985; 20(7):482-7.

PMID: 3928997 DOI: 10.1007/BF02534240.

Release of arachidonate from membrane phospholipids in cultured neonatal rat myocardial cells during adenosine triphosphate depletion. Correlation with the progression of cell injury.

Chien K, Sen A, Reynolds R, Chang A, Kim Y, Gunn M J Clin Invest. 1985; 75(6):1770-80.

PMID: 3924955 PMC: 425531. DOI: 10.1172/JCI111889.

References
1.
Scholfield C . High performance liquid chromatography of fatty methyl esters: preparative separations. Anal Chem. 1975; 47(8):1417-20. DOI: 10.1021/ac60358a063. View
2.
MOROZOWICH W, Douglas S . Resolution of prostaglandin p-nitrophenacyl esters by liquid chromatography and conditions for rapid, quantitiative p-nitrophenacylation. Prostaglandins. 1975; 10(1):19-40. DOI: 10.1016/0090-6980(75)90091-x. View
3.
Durst H, Milano M, Kikta Jr E, Connelly S, Grushka E . Phenacyl esters of fatty acids via crown ether catalysts for enhanced ultraviolet detection in liquid chromatography. Anal Chem. 1975; 47(11):1797-1801. DOI: 10.1021/ac60361a025. View
4.
Grushka E, Durst H, Kikta Jr E . Liquid chromatographic separation and detection of nanogram quantities of biologically important dicarboxylic acids. J Chromatogr. 1975; 112:673-9. DOI: 10.1016/s0021-9673(00)99996-5. View
5.
Borch R . Separation of long chain fatty acids as phenacyl esters by high pressure liquid chromatography. Anal Chem. 1975; 47(14):2437-9. DOI: 10.1021/ac60364a037. View