Detection of Methanotrophs in Groundwater by PCR

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1999 Jan 30

PMID 9925595

Citations 11

Authors

Y S Cheng

J L Halsey

K A Fode

C C Remsen

M L Collins

Affiliations

Soon will be listed here.

Abstract

Methanotrophic bacteria have significant potential for bioremediation, which would require methods for monitoring the presence and activity of these organisms in environmental samples. In this study, PCR was used to detect methanotrophic bacteria. Primers were designed on the basis of a partial sequence of pmoA, which encodes one of the proteins of the particulate methane monooxygenase. Specific amplification of a portion of pmoA was obtained with template DNA isolated from lab strains of methanotrophs. A pmoA product was also obtained by using DNA from groundwater. The identity of the PCR product was confirmed by sequencing or by amplification with a nested primer. Reverse transcriptase PCR detected pmoA mRNA.

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