Regulation and Functional Expression of Cinnamate 4-hydroxylase from Parsley

Overview

Journal Plant Physiol

Specialty Physiology

Date 1999 Jan 8

PMID 9880345

Citations 18

Authors

E Koopmann

E Logemann

K Hahlbrock

Affiliations

Soon will be listed here.

Abstract

A previously isolated parsley (Petroselinum crispum) cDNA with high sequence similarity to cinnamate 4-hydroxylase (C4H) cDNAs from several plant sources was expressed in yeast (Saccharomyces cerevisiae) containing a plant NADPH:cytochrome P450 oxidoreductase and verified as encoding a functional C4H (CYP73A10). Low genomic complexity and the occurrence of a single type of cDNA suggest the existence of only one C4H gene in parsley. The encoded mRNA and protein, in contrast to those of a functionally related NADPH:cytochrome P450 oxidoreductase, were strictly coregulated with phenylalanine ammonia-lyase mRNA and protein, respectively, as demonstrated by coinduction under various conditions and colocalization in situ in cross-sections from several different parsley tissues. These results support the hypothesis that the genes encoding the core reactions of phenylpropanoid metabolism form a tight regulatory unit.

Citing Articles

Anabolic metabolism of autotoxic substance coumarins in plants.

Wu B, Shi S, Zhang H, Lu B, Nan P, A Y PeerJ. 2023; 11:e16508.

PMID: 38077428 PMC: 10710134. DOI: 10.7717/peerj.16508.

Targeted control of supporting pathways in paclitaxel biosynthesis with CRISPR-guided methylation.

Brzycki Newton C, Young E, Roberts S Front Bioeng Biotechnol. 2023; 11:1272811.

PMID: 37915547 PMC: 10616794. DOI: 10.3389/fbioe.2023.1272811.

Functional characterization of Cinnamate 4-hydroxylase gene family in soybean (Glycine max).

Khatri P, Chen L, Rajcan I, Dhaubhadel S PLoS One. 2023; 18(5):e0285698.

PMID: 37186600 PMC: 10184913. DOI: 10.1371/journal.pone.0285698.

A Cinnamate 4-HYDROXYLASE1 from Safflower Promotes Flavonoids Accumulation and Stimulates Antioxidant Defense System in Arabidopsis.

Hou Y, Wang Y, Liu X, Ahmad N, Wang N, Jin L Int J Mol Sci. 2023; 24(6).

PMID: 36982470 PMC: 10049626. DOI: 10.3390/ijms24065393.

Comparative temporal metabolomics studies to investigate interspecies variation in three Ocimum species.

Rastogi S, Shah S, Kumar R, Kumar A, Shasany A Sci Rep. 2020; 10(1):5234.

PMID: 32251340 PMC: 7089951. DOI: 10.1038/s41598-020-61957-5.

References

Straub P, Kemper B . Deletion of a conserved tetrapeptide, PPGP, in P450 2C2 results in loss of enzymatic activity without a change in its cellular location. Arch Biochem Biophys. 1993; 304(1):170-5. DOI: 10.1006/abbi.1993.1335. View

Koopmann E, Hahlbrock K . Differentially regulated NADPH:cytochrome P450 oxidoreductases in parsley. Proc Natl Acad Sci U S A. 1998; 94(26):14954-9. PMC: 25144. DOI: 10.1073/pnas.94.26.14954. View

Logemann E, Parniske M, Hahlbrock K . Modes of expression and common structural features of the complete phenylalanine ammonia-lyase gene family in parsley. Proc Natl Acad Sci U S A. 1995; 92(13):5905-9. PMC: 41610. DOI: 10.1073/pnas.92.13.5905. View

Ma R, Cohen M, Berenbaum M, Schuler M . Black swallowtail (Papilio polyxenes) alleles encode cytochrome P450s that selectively metabolize linear furanocoumarins. Arch Biochem Biophys. 1994; 310(2):332-40. DOI: 10.1006/abbi.1994.1175. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Hahlbrock K, Knobloch K, Kreuzaler F, Potts J, Wellmann E . Coordinated induction and subsequent activity changes of two groups of metabolically interrelated enzymes. Light-induced synthesis of flavonoid glycosides in cell suspension cultures of Petroselinum hortense. Eur J Biochem. 1976; 61(1):199-206. DOI: 10.1111/j.1432-1033.1976.tb10012.x. View

Urban P, Mignotte C, Kazmaier M, Delorme F, Pompon D . Cloning, yeast expression, and characterization of the coupling of two distantly related Arabidopsis thaliana NADPH-cytochrome P450 reductases with P450 CYP73A5. J Biol Chem. 1997; 272(31):19176-86. DOI: 10.1074/jbc.272.31.19176. View

Ayers A, Ebel J, Valent B, Albersheim P . Host-Pathogen Interactions: X. Fractionation and Biological Activity of an Elicitor Isolated from the Mycelial Walls of Phytophthora megasperma var. sojae. Plant Physiol. 1976; 57(5):760-5. PMC: 542114. DOI: 10.1104/pp.57.5.760. View

Hrazdina G, Wagner G . Metabolic pathways as enzyme complexes: evidence for the synthesis of phenylpropanoids and flavonoids on membrane associated enzyme complexes. Arch Biochem Biophys. 1985; 237(1):88-100. DOI: 10.1016/0003-9861(85)90257-7. View

10.

Hotze M, Schroder G, Schroder J . Cinnamate 4-hydroxylase from Catharanthus roseus, and a strategy for the functional expression of plant cytochrome P450 proteins as translational fusions with P450 reductase in Escherichia coli. FEBS Lett. 1995; 374(3):345-50. DOI: 10.1016/0014-5793(95)01141-z. View

11.

Nelson D, Strobel H . On the membrane topology of vertebrate cytochrome P-450 proteins. J Biol Chem. 1988; 263(13):6038-50. View

12.

Durst F, OKeefe D . Plant cytochromes P450: an overview. Drug Metabol Drug Interact. 1995; 12(3-4):171-87. DOI: 10.1515/dmdi.1995.12.3-4.171. View

13.

Potter S, Moreland D, Kreuz K, Ward E . Induction of cytochrome P450 genes by ethanol in maize. Drug Metabol Drug Interact. 1995; 12(3-4):317-27. DOI: 10.1515/dmdi.1995.12.3-4.317. View

14.

Mizutani M, Ohta D . Two isoforms of NADPH:cytochrome P450 reductase in Arabidopsis thaliana. Gene structure, heterologous expression in insect cells, and differential regulation. Plant Physiol. 1998; 116(1):357-67. PMC: 35176. DOI: 10.1104/pp.116.1.357. View

15.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

16.

Hahlbrock K, Lamb C, Purwin C, Ebel J, Fautz E, Schafer E . Rapid Response of Suspension-cultured Parsley Cells to the Elicitor from Phytophthora megasperma var. sojae: INDUCTION OF THE ENZYMES OF GENERAL PHENYLPROPANOID METABOLISM. Plant Physiol. 1981; 67(4):768-73. PMC: 425770. DOI: 10.1104/pp.67.4.768. View

17.

Lindberg R, Negishi M . Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue. Nature. 1989; 339(6226):632-4. DOI: 10.1038/339632a0. View

18.

Reinold S, Hahlbrock K . Biphasic Temporal and Spatial Induction Patterns of Defense-Related mRNAs and Proteins in Fungus-Infected Parsley Leaves. Plant Physiol. 1996; 112(1):131-140. PMC: 157932. DOI: 10.1104/pp.112.1.131. View

19.

Czichi U, Kindl H . Phenylalanine ammonia lyase and cinnamic acid hydroxylases as assembled consecutive enzymes on microsomal membranes of cucumber cotyledons: Cooperation and subcellular distribution. Planta. 2014; 134(2):133-43. DOI: 10.1007/BF00384962. View

20.

Appert C, Logemann E, Hahlbrock K, Schmid J, Amrhein N . Structural and catalytic properties of the four phenylalanine ammonia-lyase isoenzymes from parsley (Petroselinum crispum Nym.). Eur J Biochem. 1994; 225(1):491-9. DOI: 10.1111/j.1432-1033.1994.00491.x. View