Membrane Filter Procedure for Enumeration of Vibrio Parahaemolyticus

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1976 Nov 1

PMID 984837

Citations 6

Authors

W D Watkins

C D Thomas

V J CABELLI

Affiliations

Soon will be listed here.

Abstract

A membrane filtration procedure has been developed for the enumeration of Vibrio parahaemolyticus in marine waters. Background microbial growth on the primary medium was decreased through the use of sodium cholate and copper sulfate, high pH, 3% NaCl, and an elevated incubation temperature. A series of in situ tests was employed to obviate the picking of colonies for identification; thereby, the enumeration of V. parahaemolyticus was accomplished within 30 h. Confirmation of typical colonies approached 95%. Relative to immediate plating on brain heart infusion agar spread plates, the recovery of V. parahaemolyticus cells suspended in phosphate-buffered saline or in seawater held for 24 h at 4 to 6 degrees C was about 90%. Assay variability did not exceed that expected by chance. Recoveries of V. parahaemolyticus from coastal and estuarine surface waters exceeded those obtainable by other methods examined.

Citing Articles

Membrane Filter Method for Enumeration of Acinetobacter calcoaceticus from Environmental Waters.

Lacroix S, CABELLI V Appl Environ Microbiol. 1982; 43(1):90-6.

PMID: 16345933 PMC: 241786. DOI: 10.1128/aem.43.1.90-96.1982.

R-plasmid transfer frequencies from environmental isolates of Escherichia coli to laboratory and fecal strains.

Shaw D, CABELLI V Appl Environ Microbiol. 1980; 40(4):756-64.

PMID: 6999996 PMC: 291656. DOI: 10.1128/aem.40.4.756-764.1980.

Effect of fecal pollution on Vibrio parahaemolyticus densities in an estuarine environment.

Watkins W, CABELLI V Appl Environ Microbiol. 1985; 49(5):1307-13.

PMID: 4004246 PMC: 238547. DOI: 10.1128/aem.49.5.1307-1313.1985.

Evaluation of four methods for enumeration of Vibrio parahaemolyticus.

DePAOLA A, Hopkins L, McPHEARSON R Appl Environ Microbiol. 1988; 54(2):617-8.

PMID: 3281584 PMC: 202508. DOI: 10.1128/aem.54.2.617-618.1988.

Membrane filter procedure for enumeration of Candida albicans in natural waters.

Buck J, Bubucis P Appl Environ Microbiol. 1978; 35(2):237-42.

PMID: 345966 PMC: 242818. DOI: 10.1128/aem.35.2.237-242.1978.

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