Peptides That Block Hepatitis B Virus Assembly: Analysis by Cryomicroscopy, Mutagenesis and Transfection

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1998 Dec 8

PMID 9843489

Citations 27

Authors

B Bottcher

N Tsuji

H Takahashi

M R Dyson

S Zhao

R A Crowther

K Murray

Affiliations

Soon will be listed here.

Abstract

Peptides selected to bind to hepatitis B virus (HBV) core protein block interaction with the long viral surface antigen (L-HBsAg) in vitro. High resolution electron cryomicroscopy showed that one such peptide binds at the tips of the spikes of the core protein shell. The peptides contain two basic residues; changing either of two acidic residues at the spike tip to an alanine greatly reduced the binding affinity. Transfection of hepatoma cells with a replication-competent HBV plasmid gave significantly reduced production of virus in the presence of peptide, in a dose-dependent manner. These experiments show that the interaction of L-HBsAg with core particles is critical for HBV assembly, and give proof of principle for its disruption in vivo by small molecules.

Citing Articles

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