Mutational Analysis of the Rhizobium Etli RecA Operator

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1998 Nov 26

PMID 9829943

Citations 5

Authors

A Tapias

J Barbe

Affiliations

Soon will be listed here.

Abstract

Based upon our earlier studies (A. Tapias, A. R. Fernández de Henestrosa, and J. Barbé, J. Bacteriol. 179:1573-1579, 1997) we hypothesized that the regulatory sequence of the Rhizobium etli recA gene was TTGN11CAA. However, further detailed analysis of the R. etli recA operator described in the present work suggests that it may in fact be GAACN7GTAC. This new conclusion is based upon PCR mutagenesis analysis carried out in the R. etli recA operator, which indicates that the GAAC and GTAC submotifs found in the sequence GAACN7GTAC are required for the maximal stimulation of in vivo transcription and in vitro DNA-protein complex formation. This DNA-protein complex is also detected when the GAACN7GTAC wild-type sequence is modified to obtain GAACN7GAAC, GTACN7GTAC, or GAACN7GTTC. The wild-type promoters of the Rhizobium meliloti and Agrobacterium tumefaciens recA genes, which also contain the GAACN7GTAC sequence, compete with the R. etli recA promoter for the DNA-protein complex formation but not with mutant derivatives in any of these motifs, indicating that the R. etli, R. meliloti, and A. tumefaciens recA genes present the same regulatory sequence.

Citing Articles

Analysis of the SOS response of Vibrio and other bacteria with multiple chromosomes.

Sanchez-Alberola N, Campoy S, Barbe J, Erill I BMC Genomics. 2012; 13:58.

PMID: 22305460 PMC: 3323433. DOI: 10.1186/1471-2164-13-58.

Prevalence of SOS-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons.

Cambray G, Sanchez-Alberola N, Campoy S, Guerin E, Da Re S, Gonzalez-Zorn B Mob DNA. 2011; 2(1):6.

PMID: 21529368 PMC: 3108266. DOI: 10.1186/1759-8753-2-6.

LexA-binding sequences in Gram-positive and cyanobacteria are closely related.

Mazon G, Lucena J, Campoy S, Fernandez de Henestrosa A, Candau P, Barbe J Mol Genet Genomics. 2003; 271(1):40-9.

PMID: 14652736 DOI: 10.1007/s00438-003-0952-x.

Definition of the mycobacterial SOS box and use to identify LexA-regulated genes in Mycobacterium tuberculosis.

Davis E, Dullaghan E, Rand L J Bacteriol. 2002; 184(12):3287-95.

PMID: 12029045 PMC: 135081. DOI: 10.1128/JB.184.12.3287-3295.2002.

Rhodobacter sphaeroides LexA has dual activity: optimising and repressing recA gene transcription.

Tapias A, Fernandez S, Alonso J, Barbe J Nucleic Acids Res. 2002; 30(7):1539-46.

PMID: 11917014 PMC: 101838. DOI: 10.1093/nar/30.7.1539.

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