The Detection by Enzyme Linked Immunosorbent Assay of P30 and 19-OH Prostaglandin F1/F2, in the Presence of a Range of Possible Contaminants

The potential of specific human semen markers, P30 and 19-OH prostaglandin F1 alpha/F2 alpha (PG F), ELISAs were compared in a blind trial. The PG F assay was shown to be at least ten times more sensitive than the P30 technique although cross reactivity problems with spermicidal creams and other possible domestic contaminants were identified. A simple method for the removal of such contaminants using a phenyl boronate column (PBA) was evaluated in an extensive blind trial.