The Major Cell Populations of the Mouse Retina

Overview

Journal J Neurosci

Specialty Neurology

Date 1998 Oct 24

PMID 9786999

Citations 718

Authors

C J Jeon

E Strettoi

R H Masland

Affiliations

Soon will be listed here.

Abstract

We report a quantitative analysis of the major populations of cells present in the retina of the C57 mouse. Rod and cone photoreceptors were counted using differential interference contrast microscopy in retinal whole mounts. Horizontal, bipolar, amacrine, and Müller cells were identified in serial section electron micrographs assembled into serial montages. Ganglion cells and displaced amacrine cells were counted by subtracting the number of axons in the optic nerve, learned from electron microscopy, from the total neurons of the ganglion cell layer. The results provide a base of reference for future work on genetically altered animals and put into perspective certain recent studies. Comparable data are now available for the retinas of the rabbit and the monkey. With the exception of the monkey fovea, the inner nuclear layers of the three species contain populations of cells that are, overall, quite similar. This contradicts the previous belief that the retinas of lower mammals are "amacrine-dominated", and therefore more complex, than those of higher mammals.

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References

Cenni M, Bonfanti L, Martinou J, Ratto G, Strettoi E, Maffei L . Long-term survival of retinal ganglion cells following optic nerve section in adult bcl-2 transgenic mice. Eur J Neurosci. 1996; 8(8):1735-45. DOI: 10.1111/j.1460-9568.1996.tb01317.x. View

Strettoi E, Masland R . The organization of the inner nuclear layer of the rabbit retina. J Neurosci. 1995; 15(1 Pt 2):875-88. PMC: 6578285. View

Masu M, Iwakabe H, Tagawa Y, Miyoshi T, Yamashita M, Fukuda Y . Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene. Cell. 1995; 80(5):757-65. DOI: 10.1016/0092-8674(95)90354-2. View

ILLING R, Wassle H . The retinal projection to the thalamus in the cat: a quantitative investigation and a comparison with the retinotectal pathway. J Comp Neurol. 1981; 202(2):265-85. DOI: 10.1002/cne.902020211. View

Gustincich S, Feigenspan A, Wu D, KOOPMAN L, Raviola E . Control of dopamine release in the retina: a transgenic approach to neural networks. Neuron. 1997; 18(5):723-36. DOI: 10.1016/s0896-6273(00)80313-x. View

Wassle H, BOYCOTT B . Functional architecture of the mammalian retina. Physiol Rev. 1991; 71(2):447-80. DOI: 10.1152/physrev.1991.71.2.447. View

Sterio D . The unbiased estimation of number and sizes of arbitrary particles using the disector. J Microsc. 1984; 134(Pt 2):127-36. DOI: 10.1111/j.1365-2818.1984.tb02501.x. View

Okano T, Kojima D, Fukada Y, Shichida Y, Yoshizawa T . Primary structures of chicken cone visual pigments: vertebrate rhodopsins have evolved out of cone visual pigments. Proc Natl Acad Sci U S A. 1992; 89(13):5932-6. PMC: 402112. DOI: 10.1073/pnas.89.13.5932. View

Williams R, Strom R, Rice D, Goldowitz D . Genetic and environmental control of variation in retinal ganglion cell number in mice. J Neurosci. 1996; 16(22):7193-205. PMC: 6578945. View

10.

Mitrofanis J, Vigny A, Stone J . Distribution of catecholaminergic cells in the retina of the rat, guinea pig, cat, and rabbit: independence from ganglion cell distribution. J Comp Neurol. 1988; 267(1):1-14. DOI: 10.1002/cne.902670102. View

11.

Wikler K, Rakic P . Distribution of photoreceptor subtypes in the retina of diurnal and nocturnal primates. J Neurosci. 1990; 10(10):3390-401. PMC: 6570186. View

12.

Nirenberg S, Meister M . The light response of retinal ganglion cells is truncated by a displaced amacrine circuit. Neuron. 1997; 18(4):637-50. DOI: 10.1016/s0896-6273(00)80304-9. View

13.

Maturana H, LETTVIN J, McCULLOCH W, Pitts W . Anatomy and physiology of vision in the frog (Rana pipiens). J Gen Physiol. 1960; 43(6)Suppl:129-75. PMC: 2195076. DOI: 10.1085/jgp.43.6.129. View

14.

Curcio C, Packer O, KALINA R . A whole mount method for sequential analysis of photoreceptor and ganglion cell topography in a single retina. Vision Res. 1987; 27(1):9-15. DOI: 10.1016/0042-6989(87)90137-4. View

15.

LaVail M . Rods and cones in the mouse retina. I. Structural analysis using light and electron microscopy. J Comp Neurol. 1979; 188(2):245-62. DOI: 10.1002/cne.901880204. View

16.

Dubin M . The inner plexiform layer of the vertebrate retina: a quantitative and comparative electron microscopic analysis. J Comp Neurol. 1970; 140(4):479-505. DOI: 10.1002/cne.901400406. View

17.

Gundersen H . Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J Microsc. 1986; 143(Pt 1):3-45. View

18.

Masland R, Rizzo 3rd J, Sandell J . Developmental variation in the structure of the retina. J Neurosci. 1993; 13(12):5194-202. PMC: 6576411. View

19.

Young R . Cell differentiation in the retina of the mouse. Anat Rec. 1985; 212(2):199-205. DOI: 10.1002/ar.1092120215. View

20.

Goldsmith T . Ultraviolet receptors and color vision: evolutionary implications and a dissonance of paradigms. Vision Res. 1994; 34(11):1479-87. DOI: 10.1016/0042-6989(94)90150-3. View