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Correlation Between Presence of Viable Bacteria and Presence of Endotoxin in Middle-ear Effusions

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1998 Oct 17
PMID 9774611
Citations 16
Authors
J R DINGMAN
M G Rayner
S Mishra
Y Zhang
M D Ehrlich
J C Post
G D Ehrlich
Affiliations
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Abstract

The presence of endotoxin (detected by the Limulus amebocyte lysate assay) was compared to the presence of viable Haemophilus influenzae and Moraxella catarrhalis (detected by PCR) in 106 middle-ear effusions from pediatric patients with chronic otitis media. Endotoxin was found in 81 of the 106 specimens. Of these 81 specimens, 66 (81.5%) also tested positive for one or both of the gram-negative bacteria H. influenzae and M. catarrhalis. The data suggest that viable gram-negative bacteria, detectable by PCR but often undetectable by culture, may be the source of endotoxin in middle-ear effusions.

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