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Kinetics of Lymphocyte Division in Blood Cultures Studied with the BrdU-Giemsa Technique

Overview
Journal Experientia
Specialty Science
Date 1976 Oct 15
PMID 976436
Citations 2
Authors
N O Bianchi
E A Lezana
Affiliations
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Abstract

Normal human lymphocytes were cultured for 72 h with different doses of BrdU. The analysis of metaphases processed with the BrdU-Giemsa method shows that in leukocyte cultures 3 different lymphocyte populations coexist which are able to perform 1, 2 or 3 rounds of replication in vitro. Moreover, it was concluded that 5 mug/ml is the minimal dose of BrdU inducing good differentiation in the areas of sister chromatid exchanges.

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Kinetics of division in PHA-stimulated pig lymphocytes.

Lezana E, Bianchi M, Bianchi N Experientia. 1978; 34(1):30-1.

PMID: 620732 DOI: 10.1007/BF01921882.

Human lymphocyte cell cycle: studies with the use of BrUdR.

auf der MAUR P Hum Genet. 1979; 49(2):209-15.

PMID: 468252 DOI: 10.1007/BF00277644.

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