Induction of IL-8 Production in Human Alveolar Macrophages and Human Bronchial Epithelial Cells in Vitro by Swine Dust

Overview

Journal Thorax

Date 1998 Sep 19

PMID 9741367

Citations 37

Authors

L Palmberg

B M Larsson

P Malmberg

K Larsson

Affiliations

Soon will be listed here.

Abstract

Background: Exposure to swine dust causes an intense airway inflammation with increased levels of interleukin 8 (IL-8) and predominantly neutrophils in the nasal and bronchoalveolar lavage fluids of healthy human subjects. It is not clear which components in the swine house environment are responsible for the airway reaction. The aim of the present study was to evaluate and compare the effect in vitro of swine dust components on human alveolar macrophages and bronchial epithelial cells.

Methods: Normal human bronchial epithelial cells (NHBE), human pulmonary epithelial carcinoma cell line (A549), and human alveolar macrophages were stimulated with swine dust, lipopolysaccharides (LPS; present in Gram negative bacteria), grain dust (swine feed components), and glucans (a structural component of fungi) in a dose response manner (1-100 micrograms/ml).

Results: Swine dust at a concentration of 100 micrograms/ml increased IL-8 production 20 fold in NHBE cells, 28 fold in A549 cells, and 15 fold in macrophages. LPS (100 micrograms/ml) stimulated all three cell types significantly, in macrophages to the same extent as swine dust, but in NHBE and A549 cells swine dust was 5-8 times as potent. Grain dust (100 micrograms/ml) had no effect in A549 cells and macrophages but not NHBE cells. Both glucans and grain dust were weaker stimuli than swine dust and LPS. The LPS content of swine dust solution was 2.16 (0.2) ng/100 micrograms and of grain dust was 0.53 (0.04) ng/100 micrograms.

Conclusions: Swine dust is a strong stimulus for IL-8 production in both bronchial epithelial cells and human alveolar macrophages, whereas LPS has different potency in these cells.

Citing Articles

The transcriptomic signature of respiratory sensitizers using an alveolar model.

Gibb M, Liu J, Sayes C Cell Biol Toxicol. 2024; 40(1):21.

PMID: 38584208 PMC: 10999393. DOI: 10.1007/s10565-024-09860-x.

An In Vitro Alveolar Model Allows for the Rapid Assessment of Particles for Respiratory Sensitization Potential.

Gibb M, Sayes C Int J Mol Sci. 2023; 24(12).

PMID: 37373252 PMC: 10298449. DOI: 10.3390/ijms241210104.

Purification and characterization of a serine protease from organic dust and elucidation of its inductive effects on lung inflammatory mediators.

Kusampudi S, Meganathan V, Keshava S, Boggaram V Am J Physiol Lung Cell Mol Physiol. 2023; 325(1):L74-L90.

PMID: 37253661 PMC: 10390052. DOI: 10.1152/ajplung.00309.2022.

Agricultural dust derived bacterial extracellular vesicle mediated inflammation is attenuated by DHA.

Heires A, Samuelson D, Villageliu D, Nordgren T, Romberger D Sci Rep. 2023; 13(1):2767.

PMID: 36797300 PMC: 9933036. DOI: 10.1038/s41598-023-29781-9.

Every breath you take: Impacts of environmental dust exposure on intestinal barrier function-from the gut-lung axis to COVID-19.

Crawford M, Nordgren T, McCole D Am J Physiol Gastrointest Liver Physiol. 2021; 320(4):G586-G600.

PMID: 33501887 PMC: 8054554. DOI: 10.1152/ajpgi.00423.2020.

References

Sandstrom T, Bjermer L, Rylander R . Lipopolysaccharide (LPS) inhalation in healthy subjects increases neutrophils, lymphocytes and fibronectin levels in bronchoalveolar lavage fluid. Eur Respir J. 1992; 5(8):992-6. View

Strieter R, Kasahara K, Allen R, Standiford T, Rolfe M, Becker F . Cytokine-induced neutrophil-derived interleukin-8. Am J Pathol. 1992; 141(2):397-407. PMC: 1886610. View

Cromwell O, Hamid Q, Corrigan C, Barkans J, Meng Q, Collins P . Expression and generation of interleukin-8, IL-6 and granulocyte-macrophage colony-stimulating factor by bronchial epithelial cells and enhancement by IL-1 beta and tumour necrosis factor-alpha. Immunology. 1992; 77(3):330-7. PMC: 1421719. View

Moller A, Lippert U, Lessmann D, Kolde G, Hamann K, Welker P . Human mast cells produce IL-8. J Immunol. 1993; 151(6):3261-6. View

Bedard M, McCLURE C, Schiller N, Francoeur C, Cantin A, Denis M . Release of interleukin-8, interleukin-6, and colony-stimulating factors by upper airway epithelial cells: implications for cystic fibrosis. Am J Respir Cell Mol Biol. 1993; 9(4):455-62. DOI: 10.1165/ajrcmb/9.4.455. View

Swensen S, Hartman T, Williams D . Computed tomographic diagnosis of Mycobacterium avium-intracellulare complex in patients with bronchiectasis. Chest. 1994; 105(1):49-52. DOI: 10.1378/chest.105.1.49. View

Massion P, Inoue H, Grunberger D, Jorens P, Housset B, Pittet J . Novel Pseudomonas product stimulates interleukin-8 production in airway epithelial cells in vitro. J Clin Invest. 1994; 93(1):26-32. PMC: 293717. DOI: 10.1172/JCI116954. View

Ruef C, Jefferson D, Suter S . Regulation of cytokine secretion by cystic fibrosis airway epithelial cells. Eur Respir J. 1993; 6(10):1429-36. View

Clapp W, Becker S, Quay J, Watt J, Thorne P, Frees K . Grain dust-induced airflow obstruction and inflammation of the lower respiratory tract. Am J Respir Crit Care Med. 1994; 150(3):611-7. DOI: 10.1164/ajrccm.150.3.8087327. View

10.

Larsson K, Eklund A, Hansson L, Isaksson B, Malmberg P . Swine dust causes intense airways inflammation in healthy subjects. Am J Respir Crit Care Med. 1994; 150(4):973-7. DOI: 10.1164/ajrccm.150.4.7921472. View

11.

Lohmann-Matthes M, Steinmuller C . Pulmonary macrophages. Eur Respir J. 1994; 7(9):1678-89. View

12.

Khair O, Devalia J, Abdelaziz M, Sapsford R, Tarraf H, Davies R . Effect of Haemophilus influenzae endotoxin on the synthesis of IL-6, IL-8, TNF-alpha and expression of ICAM-1 in cultured human bronchial epithelial cells. Eur Respir J. 1994; 7(12):2109-16. DOI: 10.1183/09031936.94.07122109. View

13.

Lieber M, Smith B, Szakal A, Todaro G . A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int J Cancer. 1976; 17(1):62-70. DOI: 10.1002/ijc.2910170110. View

14.

Zhiping W, Malmberg P, Larsson B, Larsson K, Larsson L, Saraf A . Exposure to bacteria in swine-house dust and acute inflammatory reactions in humans. Am J Respir Crit Care Med. 1996; 154(5):1261-6. DOI: 10.1164/ajrccm.154.5.8912733. View

15.

Wang Z, Larsson K, Palmberg L, Malmberg P, Larsson P, Larsson L . Inhalation of swine dust induces cytokine release in the upper and lower airways. Eur Respir J. 1997; 10(2):381-7. DOI: 10.1183/09031936.97.10020381. View

16.

Larsson B, Palmberg L, Malmberg P, Larsson K . Effect of exposure to swine dust on levels of IL-8 in airway lavage fluid. Thorax. 1997; 52(7):638-42. PMC: 1758613. DOI: 10.1136/thx.52.7.638. View

17.

Attwood P, Brouwer R, Ruigewaard P, Versloot P, de Wit R, Heederik D . A study of the relationship between airborne contaminants and environmental factors in Dutch swine confinement buildings. Am Ind Hyg Assoc J. 1987; 48(8):745-51. DOI: 10.1080/15298668791385507. View

18.

Strieter R, Kunkel S, Showell H, Remick D, Phan S, Ward P . Endothelial cell gene expression of a neutrophil chemotactic factor by TNF-alpha, LPS, and IL-1 beta. Science. 1989; 243(4897):1467-9. DOI: 10.1126/science.2648570. View

19.

SYLVESTER I, Rankin J, Yoshimura T, Tanaka S, Leonard E . Secretion of neutrophil attractant/activation protein by lipopolysaccharide-stimulated lung macrophages determined by both enzyme-linked immunosorbent assay and N-terminal sequence analysis. Am Rev Respir Dis. 1990; 141(3):683-8. DOI: 10.1164/ajrccm/141.3.683. View

20.

Rankin J, SYLVESTER I, Smith S, Yoshimura T, Leonard E . Macrophages cultured in vitro release leukotriene B4 and neutrophil attractant/activation protein (interleukin 8) sequentially in response to stimulation with lipopolysaccharide and zymosan. J Clin Invest. 1990; 86(5):1556-64. PMC: 296903. DOI: 10.1172/JCI114875. View