Protein Translocation into and Across the Chloroplastic Envelope Membranes

Overview

Journal Plant Mol Biol

Date 1998 Sep 17

PMID 9738967

Citations 25

Authors

J Soll

R Tien

Affiliations

Soon will be listed here.

Abstract

Post-translational protein import into chloroplasts follows a common route characterised by the need for nucleoside-triphosphates at various steps and two distinct protein import machineries at the outer and inner envelope membrane, respectively. Several subunits of these complexes have been elucidated. In contrast, protein translocation into the chloroplastic outer envelope uses distinct and various but poorly characterised insertion pathways. A topological framework for single-membrane spanning proteins of the chloroplastic outer envelope is presented.

Citing Articles

Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules.

Delfosse K, Wozny M, Barton K, Mathur N, Griffiths N, Mathur J Front Plant Sci. 2018; 9:754.

PMID: 29915611 PMC: 5995270. DOI: 10.3389/fpls.2018.00754.

Formation and Change of Chloroplast-Located Plant Metabolites in Response to Light Conditions.

Chen Y, Zhou B, Li J, Tang H, Tang J, Yang Z Int J Mol Sci. 2018; 19(3).

PMID: 29495387 PMC: 5877515. DOI: 10.3390/ijms19030654.

Molecular characterization and expression analysis of chloroplast protein import components in tomato (Solanum lycopersicum).

Yan J, Campbell J, Glick B, Smith M, Liang Y PLoS One. 2014; 9(4):e95088.

PMID: 24751891 PMC: 3994019. DOI: 10.1371/journal.pone.0095088.

The rice ASR5 protein: a putative role in the response to aluminum photosynthesis disturbance.

Arenhart R, Margis R, Margis-Pinheiro M Plant Signal Behav. 2012; 7(10):1263-6.

PMID: 22902685 PMC: 3493409. DOI: 10.4161/psb.21662.

Rates of evolution in stress-related genes are associated with habitat preference in two Cardamine lineages.

Ometto L, Li M, Bresadola L, Varotto C BMC Evol Biol. 2012; 12:7.

PMID: 22257588 PMC: 3398273. DOI: 10.1186/1471-2148-12-7.

References

OLSEN L, Keegstra K . The binding of precursor proteins to chloroplasts requires nucleoside triphosphates in the intermembrane space. J Biol Chem. 1992; 267(1):433-9. View

Cline K, Werner-Washburne M, Andrews J, Keegstra K . Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts. Plant Physiol. 1984; 75(3):675-8. PMC: 1066975. DOI: 10.1104/pp.75.3.675. View

Hageman J, Baecke C, Ebskamp M, Pilon R, Smeekens S, Weisbeek P . Protein Import into and Sorting inside the Chloroplast Are Independent Processes. Plant Cell. 1990; 2(5):479-494. PMC: 159904. DOI: 10.1105/tpc.2.5.479. View

Grossman A, Bartlett S, Schmidt G, Mullet J, Chua N . Optimal conditions for post-translational uptake of proteins by isolated chloroplasts. In vitro synthesis and transport of plastocyanin, ferredoxin-NADP+ oxidoreductase, and fructose-1,6-bisphosphatase. J Biol Chem. 1982; 257(3):1558-63. View

Cline K, Henry R . Import and routing of nucleus-encoded chloroplast proteins. Annu Rev Cell Dev Biol. 1996; 12:1-26. DOI: 10.1146/annurev.cellbio.12.1.1. View

OLSEN L, Theg S, Selman B, Keegstra K . ATP is required for the binding of precursor proteins to chloroplasts. J Biol Chem. 1989; 264(12):6724-9. View

Teyssier E, BLOCK M, Garin J, Joyard J, Douce R . The outer membrane protein E24 of spinach chloroplast envelope: cloning of a cDNA and topological insertion of the protein in the membrane. C R Acad Sci III. 1995; 318(1):17-25. View

von Heijne G, Gavel Y . Topogenic signals in integral membrane proteins. Eur J Biochem. 1988; 174(4):671-8. DOI: 10.1111/j.1432-1033.1988.tb14150.x. View

Fischer K, Weber A, Brink S, Arbinger B, Schunemann D, Borchert S . Porins from plants. Molecular cloning and functional characterization of two new members of the porin family. J Biol Chem. 1994; 269(41):25754-60. View

10.

Fischer K, Weber A, Arbinger B, Brink S, Eckerskorn C, Flugge U . The 24 kDa outer envelope membrane protein from spinach chloroplasts: molecular cloning, in vivo expression and import pathway of a protein with unusual properties. Plant Mol Biol. 1994; 25(2):167-77. DOI: 10.1007/BF00023235. View

11.

Theg S, Scott S . Protein import into chloroplasts. Trends Cell Biol. 1993; 3(6):186-90. DOI: 10.1016/0962-8924(93)90212-j. View

12.

Flugge U, Fischer K, Gross A, Sebald W, Lottspeich F, Eckerskorn C . The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts. EMBO J. 1989; 8(1):39-46. PMC: 400770. DOI: 10.1002/j.1460-2075.1989.tb03346.x. View

13.

Schindler C, Soll J . Protein transport in intact, purified pea etioplasts. Arch Biochem Biophys. 1986; 247(1):211-20. DOI: 10.1016/0003-9861(86)90550-3. View

14.

Pilon M, Rietveld A, Weisbeek P, de Kruijff B . Secondary structure and folding of a functional chloroplast precursor protein. J Biol Chem. 1992; 267(28):19907-13. View

15.

Li H, SULLIVAN T, Keegstra K . Information for targeting to the chloroplastic inner envelope membrane is contained in the mature region of the maize Bt1-encoded protein. J Biol Chem. 1992; 267(26):18999-9004. View

16.

GUERA A, America T, van Waas M, Weisbeek P . A strong protein unfolding activity is associated with the binding of precursor chloroplast proteins to chloroplast envelopes. Plant Mol Biol. 1993; 23(2):309-24. DOI: 10.1007/BF00029007. View

17.

Soll J . Purification and Characterization of a Chloroplast Outer-Envelope-Bound, ATP-Dependent Protein Kinase. Plant Physiol. 1988; 87(4):898-903. PMC: 1054866. DOI: 10.1104/pp.87.4.898. View

18.

Akita M, Nielsen E, Keegstra K . Identification of protein transport complexes in the chloroplastic envelope membranes via chemical cross-linking. J Cell Biol. 1997; 136(5):983-94. PMC: 2132478. DOI: 10.1083/jcb.136.5.983. View

19.

Wan J, Blakeley S, Dennis D, Ko K . Transit peptides play a major role in the preferential import of proteins into leucoplasts and chloroplasts. J Biol Chem. 1996; 271(49):31227-33. DOI: 10.1074/jbc.271.49.31227. View

20.

Brink S, Fischer K, Klosgen R, Flugge U . Sorting of nuclear-encoded chloroplast membrane proteins to the envelope and the thylakoid membrane. J Biol Chem. 1995; 270(35):20808-15. DOI: 10.1074/jbc.270.35.20808. View