A Mutation Within the Leucine-rich Repeat Domain of the Arabidopsis Disease Resistance Gene RPS5 Partially Suppresses Multiple Bacterial and Downy Mildew Resistance Genes

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1998 Sep 2

PMID 9724691

Citations 109

Authors

R F Warren

A Henk

P Mowery

E HOLUB

R W Innes

Affiliations

Soon will be listed here.

Abstract

Recognition of pathogens by plants is mediated by several distinct families of functionally variable but structurally related disease resistance (R) genes. The largest family is defined by the presence of a putative nucleotide binding domain and 12 to 21 leucine-rich repeats (LRRs). The function of these LRRs has not been defined, but they are speculated to bind pathogen-derived ligands. We have isolated a mutation in the Arabidopsis RPS5 gene that indicates that the LRR region may interact with other plant proteins. The rps5-1 mutation causes a glutamate-to-lysine substitution in the third LRR and partially compromises the function of several R genes that confer bacterial and downy mildew resistance. The third LRR is relatively well conserved, and we speculate that it may interact with a signal transduction component shared by multiple R gene pathways.

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References

Mindrinos M, Katagiri F, Yu G, Ausubel F . The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell. 1994; 78(6):1089-99. DOI: 10.1016/0092-8674(94)90282-8. View

Bisgrove S, Simonich M, Smith N, Sattler A, Innes R . A disease resistance gene in Arabidopsis with specificity for two different pathogen avirulence genes. Plant Cell. 1994; 6(7):927-33. PMC: 160489. DOI: 10.1105/tpc.6.7.927. View

Lutcke H, Chow K, Mickel F, Moss K, Kern H, SCHEELE G . Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987; 6(1):43-8. PMC: 553354. DOI: 10.1002/j.1460-2075.1987.tb04716.x. View

Martin G, Frary A, Wu T, Brommonschenkel S, Chunwongse J, Earle E . A member of the tomato Pto gene family confers sensitivity to fenthion resulting in rapid cell death. Plant Cell. 1994; 6(11):1543-52. PMC: 160542. DOI: 10.1105/tpc.6.11.1543. View

Hammond-Kosack K, Jones J . PLANT DISEASE RESISTANCE GENES. Annu Rev Plant Physiol Plant Mol Biol. 1997; 48:575-607. DOI: 10.1146/annurev.arplant.48.1.575. View

Traut T . The functions and consensus motifs of nine types of peptide segments that form different types of nucleotide-binding sites. Eur J Biochem. 1994; 222(1):9-19. DOI: 10.1111/j.1432-1033.1994.tb18835.x. View

Rommens C, Salmeron J, Baulcombe D, Staskawicz B . Use of a gene expression system based on potato virus X to rapidly identify and characterize a tomato Pto homolog that controls fenthion sensitivity. Plant Cell. 1995; 7(3):249-57. PMC: 160779. DOI: 10.1105/tpc.7.3.249. View

Kunkel B, Bent A, Dahlbeck D, Innes R, Staskawicz B . RPS2, an Arabidopsis disease resistance locus specifying recognition of Pseudomonas syringae strains expressing the avirulence gene avrRpt2. Plant Cell. 1993; 5(8):865-75. PMC: 160322. DOI: 10.1105/tpc.5.8.865. View

Hinsch M, Staskawicz B . Identification of a new Arabidopsis disease resistance locus, RPs4, and cloning of the corresponding avirulence gene, avrRps4, from Pseudomonas syringae pv. pisi. Mol Plant Microbe Interact. 1996; 9(1):55-61. DOI: 10.1094/mpmi-9-0055. View

10.

Staskawicz B, Ausubel F, Baker B, Ellis J, Jones J . Molecular genetics of plant disease resistance. Science. 1995; 268(5211):661-7. DOI: 10.1126/science.7732374. View

11.

Reuber T, Ausubel F . Isolation of Arabidopsis genes that differentiate between resistance responses mediated by the RPS2 and RPM1 disease resistance genes. Plant Cell. 1996; 8(2):241-9. PMC: 161094. DOI: 10.1105/tpc.8.2.241. View

12.

Bell C, Ecker J . Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics. 1994; 19(1):137-44. DOI: 10.1006/geno.1994.1023. View

13.

Century K, Holub E, Staskawicz B . NDR1, a locus of Arabidopsis thaliana that is required for disease resistance to both a bacterial and a fungal pathogen. Proc Natl Acad Sci U S A. 1995; 92(14):6597-601. PMC: 41565. DOI: 10.1073/pnas.92.14.6597. View

14.

Jones D, Thomas C, Hammond-Kosack K, Balint-Kurti P, Jones J . Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science. 1994; 266(5186):789-93. DOI: 10.1126/science.7973631. View

15.

Innes R, Bisgrove S, Smith N, Bent A, Staskawicz B, Liu Y . Identification of a disease resistance locus in Arabidopsis that is functionally homologous to the RPG1 locus of soybean. Plant J. 1993; 4(5):813-20. DOI: 10.1046/j.1365-313x.1993.04050813.x. View

16.

Hammond-Kosack K, Jones J . Resistance gene-dependent plant defense responses. Plant Cell. 1996; 8(10):1773-91. PMC: 161314. DOI: 10.1105/tpc.8.10.1773. View

17.

Baker B, Zambryski P, Staskawicz B, Dinesh-Kumar S . Signaling in plant-microbe interactions. Science. 1997; 276(5313):726-33. DOI: 10.1126/science.276.5313.726. View

18.

Glazebrook J, Zook M, Mert F, Kagan I, Rogers E, Crute I . Phytoalexin-deficient mutants of Arabidopsis reveal that PAD4 encodes a regulatory factor and that four PAD genes contribute to downy mildew resistance. Genetics. 1997; 146(1):381-92. PMC: 1207952. DOI: 10.1093/genetics/146.1.381. View

19.

Parker J, Holub E, Frost L, Falk A, Gunn N, Daniels M . Characterization of eds1, a mutation in Arabidopsis suppressing resistance to Peronospora parasitica specified by several different RPP genes. Plant Cell. 1996; 8(11):2033-46. PMC: 161332. DOI: 10.1105/tpc.8.11.2033. View

20.

Konieczny A, Ausubel F . A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 1993; 4(2):403-10. DOI: 10.1046/j.1365-313x.1993.04020403.x. View