Molecular Cloning of Mouse Choline Kinase and Choline/ethanolamine Kinase: Their Sequence Comparison to the Respective Rat Homologs

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 1998 Aug 26

PMID 9714798

Citations 9

Authors

C Aoyama

K Nakashima

K Ishidate

Affiliations

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Abstract

Complementary DNAs homologous to a rat 42-kDa choline/ethanolamine kinase [C. Aoyama et al., Biochim. Biophys. Acta 1390 (1998) 1-7] and to a 50-kDa choline kinase [T. Uchida and S. Yamashita, J. Biol. Chem. 267 (1992) 10156-10162] were isolated from a 17-day post coitum mouse embryo cDNA library and their sequences were compared with the two murine species, respectively. The nucleotide sequence homology (within the coding sequence) between mouse and rat 50-kDa choline kinases (96.0%) was considerably higher than that between their 42-kDa choline/ethanolamine kinases (92.4%). Northern blot and RT-PCR studies on several rat tissues demonstrated that both isozymes are expressed ubiquitously with the highest level in testis.

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