Immunological Relationship Between the Class I Epitope of Streptococcal M Protein and Myosin

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1998 Aug 26

PMID 9712796

Citations 13

Authors

A Quinn

K Ward

V A Fischetti

M Hemric

M W Cunningham

Affiliations

Soon will be listed here.

Abstract

The class I epitope of streptococcal M protein is an epidemiological marker for acute rheumatic fever (ARF)-associated serotypes of group A streptococci and is recognized by anti-M protein monoclonal antibody (MAb) 10B6. Using MAb 10B6, we determined the relationship between the class I epitope of M protein and the alpha-helical coiled-coil protein myosin. MAb 10B6 reacted by enzyme-linked immunosorbent assay and Western blotting with human cardiac myosin and rabbit skeletal myosin and its heavy meromyosin (HMM) subfragment. Overlapping synthetic peptides of M5 protein were used to identify the region of M5 protein recognized by MAb 10B6. Two C repeat peptides (C2A and C3) containing the amino acid sequence KGLRRDLDASREAK reacted with MAb 10B6. Partial sequence identity, RRDL, was found in the HMM fragment of myosin, which reacted with MAb 10B6. However, not all peptides of M5 protein and myosin containing the RRDL sequence reacted with MAb 10B6. ARF sera and sera from uncomplicated pharyngitis (UNC) reacted with C repeat region peptides of M protein, while acute glomerulonephritis sera were not as reactive. Affinity-purified human antibody to peptide C3 reacted with myosin. The data demonstrate that the class I epitope of M protein is immunologically cross-reactive with myosin and the HMM subfragment, and antibodies to peptide C3 and myosin were present in ARF and UNC sera.

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References

Krisher K, Cunningham M . Myosin: a link between streptococci and heart. Science. 1985; 227(4685):413-5. DOI: 10.1126/science.2578225. View

Pruksakorn S, Currie B, Brandt E, Phornphutkul C, Hunsakunachai S, Manmontri A . Identification of T cell autoepitopes that cross-react with the C-terminal segment of the M protein of group A streptococci. Int Immunol. 1994; 6(8):1235-44. DOI: 10.1093/intimm/6.8.1235. View

Tobacman L, Adelstein R . Enzymatic comparisons between light chain isozymes of human cardiac myosin subfragment-1. J Biol Chem. 1984; 259(18):11226-30. View

Dale J, Beachey E . Protective antigenic determinant of streptococcal M protein shared with sarcolemmal membrane protein of human heart. J Exp Med. 1982; 156(4):1165-76. PMC: 2186815. DOI: 10.1084/jem.156.4.1165. View

Bessen D, Jones K, Fischetti V . Evidence for two distinct classes of streptococcal M protein and their relationship to rheumatic fever. J Exp Med. 1989; 169(1):269-83. PMC: 2189173. DOI: 10.1084/jem.169.1.269. View

Cunningham M, Antone S, Gulizia J, McManus B, Fischetti V, Gauntt C . Cytotoxic and viral neutralizing antibodies crossreact with streptococcal M protein, enteroviruses, and human cardiac myosin. Proc Natl Acad Sci U S A. 1992; 89(4):1320-4. PMC: 48441. DOI: 10.1073/pnas.89.4.1320. View

Cunningham M, Swerlick R . Polyspecificity of antistreptococcal murine monoclonal antibodies and their implications in autoimmunity. J Exp Med. 1986; 164(4):998-1012. PMC: 2188424. DOI: 10.1084/jem.164.4.998. View

Anderson H, KUNKEL H, McCarty M . QUANTITATIVE ANTISTREPTOKINASE STUDIES IN PATIENTS INFECTED WITH GROUP A HEMOLYTIC STREPTOCOCCI: A COMPARISON WITH SERUM ANTISTREPTOLYSIN AND GAMMA GLOBULIN LEVELS WITH SPECIAL REFERENCE TO THE OCCURRENCE OF RHEUMATIC FEVER. J Clin Invest. 1948; 27(4):425-34. PMC: 439510. DOI: 10.1172/JCI101986. View

Zabriskie J, Hsu K, SEEGAL B . Heart-reactive antibody associated with rheumatic fever: characterization and diagnostic significance. Clin Exp Immunol. 1970; 7(2):147-59. PMC: 1712832. View

10.

Jones K, Khan S, Erickson B, Hollingshead S, Scott J, Fischetti V . Immunochemical localization and amino acid sequences of crossreactive epitopes within the group A streptococcal M6 protein. J Exp Med. 1986; 164(4):1226-38. PMC: 2188405. DOI: 10.1084/jem.164.4.1226. View

11.

Cunningham M, Antone S, Smart M, Liu R, Kosanke S . Molecular analysis of human cardiac myosin-cross-reactive B- and T-cell epitopes of the group A streptococcal M5 protein. Infect Immun. 1997; 65(9):3913-23. PMC: 175558. DOI: 10.1128/iai.65.9.3913-3923.1997. View

12.

Miller L, Gray L, Beachey E, Kehoe M . Antigenic variation among group A streptococcal M proteins. Nucleotide sequence of the serotype 5 M protein gene and its relationship with genes encoding types 6 and 24 M proteins. J Biol Chem. 1988; 263(12):5668-73. View

13.

Jones K, Fischetti V . Biological and immunochemical identity of M protein on group G streptococci with M protein on group A streptococci. Infect Immun. 1987; 55(3):502-6. PMC: 260364. DOI: 10.1128/iai.55.3.502-506.1987. View

14.

Dell A, Antone S, Gauntt C, Crossley C, Clark W, Cunningham M . Autoimmune determinants of rheumatic carditis: localization of epitopes in human cardiac myosin. Eur Heart J. 1991; 12 Suppl D:158-62. DOI: 10.1093/eurheartj/12.suppl_d.158. View

15.

Jaenicke T, DIEDERICH K, Haas W, Schleich J, Lichter P, Pfordt M . The complete sequence of the human beta-myosin heavy chain gene and a comparative analysis of its product. Genomics. 1990; 8(2):194-206. DOI: 10.1016/0888-7543(90)90272-v. View

16.

Liao L, Sindhwani R, Rojkind M, Factor S, Leinwand L, Diamond B . Antibody-mediated autoimmune myocarditis depends on genetically determined target organ sensitivity. J Exp Med. 1995; 181(3):1123-31. PMC: 2191921. DOI: 10.1084/jem.181.3.1123. View

17.

Manjula B, Trus B, Fischetti V . Presence of two distinct regions in the coiled-coil structure of the streptococcal Pep M5 protein: relationship to mammalian coiled-coil proteins and implications to its biological properties. Proc Natl Acad Sci U S A. 1985; 82(4):1064-8. PMC: 397194. DOI: 10.1073/pnas.82.4.1064. View

18.

Collins C, Kimura A, BISNO A . Group G streptococcal M protein exhibits structural features analogous to those of class I M protein of group A streptococci. Infect Immun. 1992; 60(9):3689-96. PMC: 257378. DOI: 10.1128/iai.60.9.3689-3696.1992. View

19.

Cunningham M, McCormack J, Talaber L, Harley J, Ayoub E, Muneer R . Human monoclonal antibodies reactive with antigens of the group A Streptococcus and human heart. J Immunol. 1988; 141(8):2760-6. View

20.

Adderson E, Shikhman A, Ward K, Cunningham M . Molecular analysis of polyreactive monoclonal antibodies from rheumatic carditis: human anti-N-acetylglucosamine/anti-myosin antibody V region genes. J Immunol. 1998; 161(4):2020-31. View