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The Nucleolus is the Site of Borna Disease Virus RNA Transcription and Replication

Overview
Journal J Virol
Publisher American Society for Microbiology
Date 1998 Aug 8
PMID 9696879
Citations 32
Authors
J M Pyper
J E Clements
M C Zink
Affiliations
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Abstract

Borna disease virus (BDV) is a neurotropic nonsegmented negative-strand RNA virus with limited homology to rhabdoviruses and paramyxoviruses. A distinguishing feature of BDV is that it replicates in the nucleus of infected cells. Strand-specific probes used for in situ hybridization of infected rat brain showed that there was differential localization of positive- and negative-strand RNAs within the nucleus of neurons. Within nuclei, sense-strand RNAs were preferentially localized within nucleolar regions while genomic-sense RNAs were found in both nucleolar and nonnucleolar regions. These results suggested a role for the nucleolus in BDV replication. Nucleoli isolated from persistently infected neuroblastoma cells contained both genomic and antigenomic BDV RNA species as well as an enrichment of the 39/38-kDa and gp18 BDV proteins. Since the nucleolus is the site of rRNA transcription, we examined BDV transcription in the presence of inhibitors of RNA polymerase I. Inhibition of RNA polymerase I did not affect levels of BDV transcription.

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References
1.
Richt J, Vande Woude S, Zink M, Narayan O, Clements J . Analysis of Borna disease virus-specific RNAs in infected cells and tissues. J Gen Virol. 1991; 72 ( Pt 9):2251-5. DOI: 10.1099/0022-1317-72-9-2251. View
2.
Carmo-Fonseca M, Pepperkok R, Sproat B, Ansorge W, Swanson M, Lamond A . In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells. EMBO J. 1991; 10(7):1863-73. PMC: 452861. DOI: 10.1002/j.1460-2075.1991.tb07712.x. View
3.
Gdovin S, Clements J . Molecular mechanisms of visna virus Tat: identification of the targets for transcriptional activation and evidence for a post-transcriptional effect. Virology. 1992; 188(2):438-50. DOI: 10.1016/0042-6822(92)90497-d. View
4.
Briese T, de la Torre J, Lewis A, Ludwig H, Lipkin W . Borna disease virus, a negative-strand RNA virus, transcribes in the nucleus of infected cells. Proc Natl Acad Sci U S A. 1992; 89(23):11486-9. PMC: 50576. DOI: 10.1073/pnas.89.23.11486. View
5.
Adachi Y, Copeland T, Hatanaka M, Oroszlan S . Nucleolar targeting signal of Rex protein of human T-cell leukemia virus type I specifically binds to nucleolar shuttle protein B-23. J Biol Chem. 1993; 268(19):13930-4. View