TAFII-independent Activation Mediated by Human TBP in the Presence of the Positive Cofactor PC4

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1998 Aug 4

PMID 9687514

Citations 24

Authors

S Y Wu

E Kershnar

C M Chiang

Affiliations

Soon will be listed here.

Abstract

TFIID is a multiprotein complex comprised of the TATA-binding protein (TBP) and an array of TBP-associated factors (TAFIIs). Whereas TBP is sufficient for basal transcription in conjunction with other general transcription factors and RNA polymerase II, TAFIIs are additionally required for activator-dependent transcription in mammalian cell-free transcription systems. However, recent in vivo studies carried out in yeast suggest that TAFIIs are not globally required for activator function. The discrepancy between in vivo yeast studies and in vitro mammalian cell-free systems remains to be resolved. In this study, we describe a mammalian cell-free transcription system reconstituted with only recombinant proteins and epitope-tagged multiprotein complexes. Transcriptional activation can be recapitulated in this highly purified in vitro transcription system in the absence of TAFIIs. This TBP-mediated activation is not induced by human mediator, another transcriptional coactivator complex potentially implicated in activator response. In contrast, general transcription factors TFIIH and TFIIA play a significant role in TBP-mediated activation, which can be detected in vitro with Gal4 fusion proteins containing various transcriptional activation domains. Our data, therefore, suggest that TFIIH and TFIIA can mediate activator function in the absence of TAFIIs.

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References

Kingston R, Bunker C, Imbalzano A . Repression and activation by multiprotein complexes that alter chromatin structure. Genes Dev. 1996; 10(8):905-20. DOI: 10.1101/gad.10.8.905. View

Maldonado E, Shiekhattar R, Sheldon M, Cho H, Drapkin R, Rickert P . A human RNA polymerase II complex associated with SRB and DNA-repair proteins. Nature. 1996; 381(6577):86-9. DOI: 10.1038/381086a0. View

Choy B, Green M . Eukaryotic activators function during multiple steps of preinitiation complex assembly. Nature. 1993; 366(6455):531-6. DOI: 10.1038/366531a0. View

Kretzschmar M, Meisterernst M, Roeder R . Identification of human DNA topoisomerase I as a cofactor for activator-dependent transcription by RNA polymerase II. Proc Natl Acad Sci U S A. 1993; 90(24):11508-12. PMC: 48013. DOI: 10.1073/pnas.90.24.11508. View

Wang E, Tjian R . Promoter-selective transcriptional defect in cell cycle mutant ts13 rescued by hTAFII250. Science. 1994; 263(5148):811-4. DOI: 10.1126/science.8303298. View

Timmers H . Transcription initiation by RNA polymerase II does not require hydrolysis of the beta-gamma phosphoanhydride bond of ATP. EMBO J. 1994; 13(2):391-9. PMC: 394820. DOI: 10.1002/j.1460-2075.1994.tb06273.x. View

Koleske A, Young R . An RNA polymerase II holoenzyme responsive to activators. Nature. 1994; 368(6470):466-9. DOI: 10.1038/368466a0. View

Buratowski S . The basics of basal transcription by RNA polymerase II. Cell. 1994; 77(1):1-3. DOI: 10.1016/0092-8674(94)90226-7. View

Goodrich J, Tjian R . Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II. Cell. 1994; 77(1):145-56. DOI: 10.1016/0092-8674(94)90242-9. View

10.

Kim Y, Bjorklund S, Li Y, Sayre M, Kornberg R . A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. Cell. 1994; 77(4):599-608. DOI: 10.1016/0092-8674(94)90221-6. View

11.

Ge H, Roeder R . The high mobility group protein HMG1 can reversibly inhibit class II gene transcription by interaction with the TATA-binding protein. J Biol Chem. 1994; 269(25):17136-40. View

12.

Kraus V, Inostroza J, Yeung K, Reinberg D, Nevins J . Interaction of the Dr1 inhibitory factor with the TATA binding protein is disrupted by adenovirus E1A. Proc Natl Acad Sci U S A. 1994; 91(14):6279-82. PMC: 44184. DOI: 10.1073/pnas.91.14.6279. View

13.

Parvin J, Shykind B, Meyers R, Kim J, Sharp P . Multiple sets of basal factors initiate transcription by RNA polymerase II. J Biol Chem. 1994; 269(28):18414-21. View

14.

Ding H, Rimsky S, Batson S, Bustin M, Hansen U . Stimulation of RNA polymerase II elongation by chromosomal protein HMG-14. Science. 1994; 265(5173):796-9. DOI: 10.1126/science.8047885. View

15.

Ge H, Roeder R . Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes. Cell. 1994; 78(3):513-23. DOI: 10.1016/0092-8674(94)90428-6. View

16.

Kretzschmar M, Kaiser K, Lottspeich F, Meisterernst M . A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators. Cell. 1994; 78(3):525-34. DOI: 10.1016/0092-8674(94)90429-4. View

17.

Xiao H, Pearson A, Coulombe B, Truant R, Zhang S, Regier J . Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53. Mol Cell Biol. 1994; 14(10):7013-24. PMC: 359231. DOI: 10.1128/mcb.14.10.7013-7024.1994. View

18.

Reese J, Apone L, Walker S, Griffin L, Green M . Yeast TAFIIS in a multisubunit complex required for activated transcription. Nature. 1994; 371(6497):523-7. DOI: 10.1038/371523a0. View

19.

Yokomori K, Zeidler M, Chen J, Verrijzer C, Mlodzik M, Tjian R . Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation. Genes Dev. 1994; 8(19):2313-23. DOI: 10.1101/gad.8.19.2313. View

20.

Ozer J, Moore P, BOLDEN A, Lee A, Rosen C, Lieberman P . Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription. Genes Dev. 1994; 8(19):2324-35. DOI: 10.1101/gad.8.19.2324. View