The Yeast Galactose Genetic Switch is Mediated by the Formation of a Gal4p-Gal80p-Gal3p Complex

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1998 Jul 22

PMID 9670023

Citations 75

Authors

A Platt

R J Reece

Affiliations

Soon will be listed here.

Abstract

Saccharomyces cerevisiae responds to galactose as the sole source of carbon by activating the GAL genes encoding the enzymes of the Leloir pathway. Here, we show in vitro that the switch from repressed to activated gene expression involves the interplay of three proteins [an activator (Gal4p), a repressor (Gal80p) and an inducer (Gal3p)] and two small molecules (galactose and ATP). We also show that the galactose- and ATP-dependent interaction between Gal3p and Gal80p occurs without disruption of the Gal80p-Gal4p interaction. Thus, Gal3p-mediated activation of transcription occurs via the formation of a tripartite protein complex.

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