Cytogenetic and FISH Analyses of Pancreatic Carcinoma Reveal Breaks in 18q11 with Consistent Loss of 18q12-qter and Frequent Gain of 18p

Overview

Journal Br J Cancer

Specialty Oncology

Date 1998 Jul 17

PMID 9667665

Citations 2

Authors

M Hoglund

L Gorunova

T Jonson

S Dawiskiba

A Andren-Sandberg

G Stenman

B Johansson

Affiliations

Soon will be listed here.

Abstract

Chromosome 18 was analysed using a banding technique and fluorescence in situ hybridization (FISH) in 13 pancreatic carcinoma samples. The cytogenetic analysis revealed that chromosome 18 abnormalities were present in all cases and that several different rearrangements, such as translocations, deletions, dicentrics and ring chromosomes, were often found together. FISH mapping using 18q YAC probes showed that all tumours had lost at least one copy of 18q and that 18p was over-represented in 6 of the 13 cases. Furthermore, out of 13 identified deletion breakpoints on 18q, 11 were mapped to 18q11. The clustering of breaks close to the centromere indicates that loss of genes in bands 18q11 and 18q12, in addition to those located in 18q21, e.g. DPC4 and DCC, are important in the development of pancreatic tumours.

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References

Fearon E, Cho K, Nigro J, Kern S, Simons J, Ruppert J . Identification of a chromosome 18q gene that is altered in colorectal cancers. Science. 1990; 247(4938):49-56. DOI: 10.1126/science.2294591. View

Johansson B, Bardi G, Heim S, Mandahl N, Mertens F, Andren-Sandberg A . Nonrandom chromosomal rearrangements in pancreatic carcinomas. Cancer. 1992; 69(7):1674-81. DOI: 10.1002/1097-0142(19920401)69:7<1674::aid-cncr2820690706>3.0.co;2-l. View

Hohne M, Halatsch M, Kahl G, Weinel R . Frequent loss of expression of the potential tumor suppressor gene DCC in ductal pancreatic adenocarcinoma. Cancer Res. 1992; 52(9):2616-9. View

Ruggeri B, Zhang S, Caamano J, DiRado M, Flynn S, Klein-Szanto A . Human pancreatic carcinomas and cell lines reveal frequent and multiple alterations in the p53 and Rb-1 tumor-suppressor genes. Oncogene. 1992; 7(8):1503-11. View

Lengauer C, Green E, Cremer T . Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification. Genomics. 1992; 13(3):826-8. DOI: 10.1016/0888-7543(92)90160-t. View

Silverman G, Kuo W, Taillon-Miller P, Gray J . Chromosomal reassignment: YACs containing both YES1 and thymidylate synthase map to the short arm of chromosome 18. Genomics. 1993; 15(2):442-5. DOI: 10.1006/geno.1993.1086. View

Scarpa A, Capelli P, Mukai K, Zamboni G, Oda T, Iacono C . Pancreatic adenocarcinomas frequently show p53 gene mutations. Am J Pathol. 1993; 142(5):1534-43. PMC: 1886920. View

Bardi G, Johansson B, Pandis N, Mandahl N, Andren-Sandberg A, Mitelman F . Karyotypic abnormalities in tumours of the pancreas. Br J Cancer. 1993; 67(5):1106-12. PMC: 1968474. DOI: 10.1038/bjc.1993.203. View

Park J, Meisler A, Cartwright C . c-Yes tyrosine kinase activity in human colon carcinoma. Oncogene. 1993; 8(10):2627-35. View

10.

Loganzo Jr F, Dosik J, Zhao Y, Vidal M, Nanus D, Sudol M . Elevated expression of protein tyrosine kinase c-Yes, but not c-Src, in human malignant melanoma. Oncogene. 1993; 8(10):2637-44. View

11.

Seymour A, Hruban R, Redston M, Caldas C, Powell S, Kinzler K . Allelotype of pancreatic adenocarcinoma. Cancer Res. 1994; 54(10):2761-4. View

12.

Simon B, Weinel R, Hohne M, Watz J, Schmidt J, Kortner G . Frequent alterations of the tumor suppressor genes p53 and DCC in human pancreatic carcinoma. Gastroenterology. 1994; 106(6):1645-51. DOI: 10.1016/0016-5085(94)90422-7. View

13.

Caldas C, Hahn S, DA COSTA L, Redston M, Schutte M, Seymour A . Frequent somatic mutations and homozygous deletions of the p16 (MTS1) gene in pancreatic adenocarcinoma. Nat Genet. 1994; 8(1):27-32. DOI: 10.1038/ng0994-27. View

14.

Griffin C, Hruban R, Morsberger L, Ellingham T, Long P, Jaffee E . Consistent chromosome abnormalities in adenocarcinoma of the pancreas. Cancer Res. 1995; 55(11):2394-9. View

15.

Voorter C, Joos S, Bringuier P, Vallinga M, Poddighe P, Schalken J . Detection of chromosomal imbalances in transitional cell carcinoma of the bladder by comparative genomic hybridization. Am J Pathol. 1995; 146(6):1341-54. PMC: 1870895. View

16.

Hahn S, Seymour A, Hoque A, Schutte M, DA COSTA L, Redston M . Allelotype of pancreatic adenocarcinoma using xenograft enrichment. Cancer Res. 1995; 55(20):4670-5. View

17.

Hahn S, Schutte M, Hoque A, Moskaluk C, DA COSTA L, Rozenblum E . DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1. Science. 1996; 271(5247):350-3. DOI: 10.1126/science.271.5247.350. View

18.

Bartsch D, Shevlin D, Tung W, Kisker O, Wells Jr S, Goodfellow P . Frequent mutations of CDKN2 in primary pancreatic adenocarcinomas. Genes Chromosomes Cancer. 1995; 14(3):189-95. DOI: 10.1002/gcc.2870140306. View

19.

Gorunova L, Johansson B, Dawiskiba S, Andren-Sandberg A, Jin Y, Mandahl N . Massive cytogenetic heterogeneity in a pancreatic carcinoma: fifty-four karyotypically unrelated clones. Genes Chromosomes Cancer. 1995; 14(4):259-66. DOI: 10.1002/gcc.2870140404. View

20.

Schutte M, Hruban R, Hedrick L, Cho K, Nadasdy G, Weinstein C . DPC4 gene in various tumor types. Cancer Res. 1996; 56(11):2527-30. View