Outer Membrane Profiles of Clonally Related Klebsiella Pneumoniae Isolates from Clinical Samples and Activities of Cephalosporins and Carbapenems

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1998 Jul 14

PMID 9660996

Citations 38

Authors

C Ardanuy

J Linares

M A Dominguez

V J Benedi

L Martinez-Martinez

Affiliations

Soon will be listed here.

Abstract

Fifteen isolates of Klebsiella pneumoniae producing extended-spectrum beta-lactamases (ESBLs) isolated during a nosocomial outbreak were studied. The strains belonged to the same clonal type, as shown by pulsed-field gel electrophoretic analysis of chromosomal DNA. All the isolates were resistant to extended-spectrum cephalosporins, aztreonam, gentamicin, and fluoroquinolones and were susceptible to carbapenems, tobramycin, netilmicin, and amikacin. None of the isolates expressed the OmpK36 porin. Eight isolates, for which the MICs of cefoxitin were > or = 64 micrograms/ml, showed a diminished level or no expression of a 35-kDa porin. The MICs of meropenem, cefotaxime, and cefpirome were three to eight times higher for porin-deficient isolates than for isolates expressing the 35-kDa porin, but the MICs of imipenem increased two times for porin-deficient isolates compared to those for isolates expressing the porin. This MIC increase reverted to a level similar to that for the parental strain when porin-deficient isolates were transformed with the gene coding for the K. pneumoniae porin OmpK36. It is concluded that the high level of resistance to cefoxitin and the increase in the MICs of meropenem, cefotaxime, and cefpirome for the ESBL-producing K. pneumoniae isolates studied are associated with porin deficiency.

Citing Articles

Resistome Analysis of Complex from Residential Aged Care Facilities Demonstrates Intra-facility Clonal Spread of Multidrug-Resistant Isolates.

Blaikie J, Sapula S, Siderius N, Hart B, Amsalu A, Leong L Microorganisms. 2024; 12(4).

PMID: 38674695 PMC: 11051875. DOI: 10.3390/microorganisms12040751.

Development of Meropenem Resistance in a Multidrug-Resistant Campylobacter coli Strain Causing Recurrent Bacteremia in a Hematological Malignancy Patient.

Bonilla-Moreno M, Torrecillas M, Laporte-Amargos J, Gonzalez-Diaz A, Mussetti A, Tubau F Antimicrob Agents Chemother. 2023; 67(11):e0027223.

PMID: 37358413 PMC: 10648861. DOI: 10.1128/aac.00272-23.

Evaluation of the Organotellurium Compound AS101 for Treating Colistin- and Carbapenem-Resistant .

Yang T, Kao H, Lu P, Chen P, Wang S, Wang L Pharmaceuticals (Basel). 2021; 14(8).

PMID: 34451891 PMC: 8400984. DOI: 10.3390/ph14080795.

Genomic evolution and local epidemiology of from a major hospital in Beijing, China, over a 15 year period: dissemination of known and novel high-risk clones.

Palmieri M, Wyres K, Mirande C, Qiang Z, Liyan Y, Gang C Microb Genom. 2021; 7(6).

PMID: 33629946 PMC: 8627660. DOI: 10.1099/mgen.0.000520.

Emergence of the New KPC-49 Variant Conferring an ESBL Phenotype with Resistance to Ceftazidime-Avibactam in the ST131-H30R1 High-Risk Clone.

Hernandez-Garcia M, Sanchez-Lopez J, Martinez-Garcia L, Becerra-Aparicio F, Morosini M, Ruiz-Garbajosa P Pathogens. 2021; 10(1).

PMID: 33466574 PMC: 7828710. DOI: 10.3390/pathogens10010067.

References

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Pena C, Pujol M, Ardanuy C, Ricart A, Pallares R, Linares J . Epidemiology and successful control of a large outbreak due to Klebsiella pneumoniae producing extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1998; 42(1):53-8. PMC: 105455. DOI: 10.1128/AAC.42.1.53. View

Gutmann L, Williamson R, Moreau N, Kitzis M, Collatz E, Acar J . Cross-resistance to nalidixic acid, trimethoprim, and chloramphenicol associated with alterations in outer membrane proteins of Klebsiella, Enterobacter, and Serratia. J Infect Dis. 1985; 151(3):501-7. DOI: 10.1093/infdis/151.3.501. View

Brun-Buisson C, Legrand P, Philippon A, Montravers F, Ansquer M, Duval J . Transferable enzymatic resistance to third-generation cephalosporins during nosocomial outbreak of multiresistant Klebsiella pneumoniae. Lancet. 1987; 2(8554):302-6. DOI: 10.1016/s0140-6736(87)90891-9. View

van de Klundert J, van Gestel M, Meerdink G, de Marie S . Emergence of bacterial resistance to cefamandole in vivo due to outer membrane protein deficiency. Eur J Clin Microbiol Infect Dis. 1988; 7(6):776-8. DOI: 10.1007/BF01975046. View

Elhai J, Wolk C . A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene. 1988; 68(1):119-38. DOI: 10.1016/0378-1119(88)90605-1. View

Pangon B, Bizet C, Bure A, Pichon F, Philippon A, Regnier B . In vivo selection of a cephamycin-resistant, porin-deficient mutant of Klebsiella pneumoniae producing a TEM-3 beta-lactamase. J Infect Dis. 1989; 159(5):1005-6. DOI: 10.1093/infdis/159.5.1005. View

Legrand P, Fournier G, Bure A, Jarlier V, Nicolas M, Decre D . Detection of extended broad-spectrum beta-lactamases in Enterobacteriaceae in four French hospitals. Eur J Clin Microbiol Infect Dis. 1989; 8(6):527-9. DOI: 10.1007/BF01967473. View

Nikaido H . Outer membrane barrier as a mechanism of antimicrobial resistance. Antimicrob Agents Chemother. 1989; 33(11):1831-6. PMC: 172772. DOI: 10.1128/AAC.33.11.1831. View

10.

Papanicolaou G, Medeiros A, Jacoby G . Novel plasmid-mediated beta-lactamase (MIR-1) conferring resistance to oxyimino- and alpha-methoxy beta-lactams in clinical isolates of Klebsiella pneumoniae. Antimicrob Agents Chemother. 1990; 34(11):2200-9. PMC: 172023. DOI: 10.1128/AAC.34.11.2200. View

11.

Raimondi A, Traverso A, Nikaido H . Imipenem- and meropenem-resistant mutants of Enterobacter cloacae and Proteus rettgeri lack porins. Antimicrob Agents Chemother. 1991; 35(6):1174-80. PMC: 284306. DOI: 10.1128/AAC.35.6.1174. View

12.

Jacoby G, Medeiros A . More extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1991; 35(9):1697-704. PMC: 245253. DOI: 10.1128/AAC.35.9.1697. View

13.

Jeanteur D, Lakey J, Pattus F . The bacterial porin superfamily: sequence alignment and structure prediction. Mol Microbiol. 1991; 5(9):2153-64. DOI: 10.1111/j.1365-2958.1991.tb02145.x. View

14.

Meyer K, Urban C, Eagan J, Berger B, Rahal J . Nosocomial outbreak of Klebsiella infection resistant to late-generation cephalosporins. Ann Intern Med. 1993; 119(5):353-8. DOI: 10.7326/0003-4819-119-5-199309010-00001. View

15.

Chen H, Livermore D . Activity of cefepime and other beta-lactam antibiotics against permeability mutants of Escherichia coli and Klebsiella pneumoniae. J Antimicrob Chemother. 1993; 32 Suppl B:63-74. DOI: 10.1093/jac/32.suppl_b.63. View

16.

Gouby A, Neuwirth C, Bourg G, Bouziges N, Despaux E, Ramuz M . Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a geriatric hospital. J Clin Microbiol. 1994; 32(2):301-5. PMC: 263028. DOI: 10.1128/jcm.32.2.301-305.1994. View

17.

Gonzalez Leiza M, Perez-Diaz J, Ayala J, Casellas J, Bush K, Baquero F . Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants. Antimicrob Agents Chemother. 1994; 38(9):2150-7. PMC: 284699. DOI: 10.1128/AAC.38.9.2150. View

18.

Alberti S, Schirmer T, Rummel G, Tomas J, Rosenbusch J, Benedi V . A porin from Klebsiella pneumoniae: sequence homology, three-dimensional model, and complement binding. Infect Immun. 1995; 63(3):903-10. PMC: 173088. DOI: 10.1128/iai.63.3.903-910.1995. View

19.

Cornaglia G, Russell K, Satta G, Fontana R . Relative importances of outer membrane permeability and group 1 beta-lactamase as determinants of meropenem and imipenem activities against Enterobacter cloacae. Antimicrob Agents Chemother. 1995; 39(2):350-5. PMC: 162541. DOI: 10.1128/AAC.39.2.350. View

20.

Morosini M, Canton R, Negri M, Perez-Diaz J, Baquero F, Blazquez J . New extended-spectrum TEM-type beta-lactamase from Salmonella enterica subsp. enterica isolated in a nosocomial outbreak. Antimicrob Agents Chemother. 1995; 39(2):458-61. PMC: 162560. DOI: 10.1128/AAC.39.2.458. View