Hepatitis B Antigen-associated Deoxyribonucleic Acid Polymerase Activity and E Antigen/anti-e System

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1976 Sep 1

PMID 965088

Citations 16

Authors

M Imai

F C Tachibana

Y Moritsugu

Y Miyakawa

M Mayumi

Affiliations

Soon will be listed here.

Abstract

Serum samples of 403 asymptomatic blood donors carrying hepatitis B surface antigen (HB5Ag) were concentrated threefold and tested for e antigen and antibody to e antigen (anti-e) by immunodiffusion. Hepatitis B antigen (HBAg)-associated deoxyribonucleic acid (DNA) polymerase activity was specifically determined by the difference in incorporation of [methyl-3H]thymidine 5' -triphosphate into DNA by an aliquot of centrifuged serum samples after it had been treated either with normal rabbit serum or with rabbit antibody to HBSAg. All of 58 serum samples containing e antigen revealed HBAg-associated DNA polymerase activity, whereas none of 96 samples containing anti-e did. In the remaining 249 samples in which neither e antigen nor anti-e was found, 62 showed specific DNA polymerase activity, although at lower levels than the samples containing e antigen.

Citing Articles

Comparison of indicators for dane particles.

Hess G, Arnold W, Meyer zum Buschenfelde K Klin Wochenschr. 1980; 58(7):371-6.

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Hepatitis B e antigen and antibody in asymptomatic Chinese with hepatitis B surface antigenemia in Taiwan.

SUNG J, Chen D, Lai M, Wang T, Wang C, Yu J Gastroenterol Jpn. 1982; 17(4):341-6.

PMID: 7141198 DOI: 10.1007/BF02774580.

Hepatitis B e-antigen titres for evaluating infectivity during hepatitis B virus infection.

Lindenschmidt E, Granato C, Salefsky C, Laufs R, Henning H Klin Wochenschr. 1984; 62(5):231-7.

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Hepatitis B virus DNA polymerase activity and hepatitis B e antigen (HBeAg)/anti-HBe status among type B chronic liver diseases.

Kashiwagi S, Hayashi J, Ikematsu H, Nomura H, Kajiyama W, Shingu T Gastroenterol Jpn. 1988; 23(2):117-23.

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Mother to child transmission of hepatitis B virus in the Philippines.

Lingao A, Torres N, Munoz N, Lansang M, West S, Bosch F Infection. 1989; 17(5):275-9.

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