Characteristics of Ribonucleic Acids Isolated for Botryodiplodia Theobromae Pycnidiospores

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 1976 Aug 1

PMID 962472

Citations 5

Authors

R H Knight

J L Van Etten

Affiliations

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Abstract

Nucleic acids isolated for dormant and germinated Botryodiplodia theobromae pycnidiospores contain five distinct species of RNA. They include two ribosomal species, two "ribosomal-associated" species and transfer RNAs. Sedimentation coefficients of 25.1S and 18S were obtained for the two ribosomal RNA species and 5.8S and 5S for the two "ribosomal-associated" RNA components. Molecular weights of 1.20,0.67,0.054 and 0.035x106 daltons were obtained after formaldehyde treatment and electrophoresis on polyacrylamide gels for these same four RNAs. Methylated nucleotides were present in the transfer RNAs and large and small ribosomal RNAs; in contrast 5.8S and 5S RNAs contained few methylated nucleotides. In addition to the 5 distinct RNA species, polyadenylate-containing RNA was isolated from both dormant and germinated spores.

Citing Articles

Pattern of ribonucleic acid synthesis during germination of Allomyces macrogynus mitospores.

Smith B, Burke D J Bacteriol. 1980; 143(3):1353-61.

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Stored mRNA in sporangiospores of the fungus Mucor racemosus.

Linz J, Orlowski M J Bacteriol. 1982; 150(3):1138-44.

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Properties of polyadenylate-associated ribonucleic acid from Saccharomyces cerevisiae ascospores.

Harper J, Clancy M, Magee P J Bacteriol. 1980; 143(2):958-65.

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Evidence for the presence of messenger ribonucleic acid in Allomyces macrogynus mitospores.

Smith B, Burke D J Bacteriol. 1979; 138(2):535-41.

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