Detection of Circulating Paracoccidioides Brasiliensis Antigen in Urine of Paracoccidioidomycosis Patients Before and During Treatment

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1998 Jun 10

PMID 9620407

Citations 15

Authors

M A Salina

M A Shikanai-Yasuda

R P Mendes

B Barraviera

M J Mendes Giannini

Affiliations

Soon will be listed here.

Abstract

For the diagnosis and follow-up of paracoccidioidomycosis patients undergoing therapy, we evaluated two methods (immunoblotting and competition enzyme immunoassay) for the detection of circulating antigen in urine samples. A complex pattern of reactivity was observed in the immunoblot test. Bands of 70 and 43 kDa were detected more often in urine samples from patients before treatment. The immunoblot method detected gp43 and gp70 separately or concurrently in 11 (91.7%) of 12 patients, whereas the competition enzyme immunoassay detected antigenuria in 9 (75%) of 12 patients. Both tests appeared to be highly specific (100%), considering that neither fraction detectable by immunoblotting was present in urine samples from the control group. gp43 remained present in the urine samples collected during the treatment period, with a significant decrease in reactivity in samples collected during clinical recovery and increased reactivity in samples collected during relapses. Reactivity of some bands was also detected in urine specimens from patients with "apparent cure." The detection of Paracoccidioides brasiliensis antigens in urine appears to be a promising method for diagnosing infection, for evaluating the efficacy of treatment, and for detecting relapse.

Citing Articles

Standardization of Semi-Quantitative Dot Blotting Assay-Application in the Diagnosis, Follow-Up, and Relapse of Paracoccidioidomycosis.

Pereira B, Cavalcante R, Pereira-Chioccola V, de Souza Carvalho Melhem M, de Carvalho L, Mendes R Microorganisms. 2024; 12(2).

PMID: 38399756 PMC: 10892552. DOI: 10.3390/microorganisms12020351.

Insights into Magnetic Resonance Imaging Findings in Central Nervous System Paracoccidioidomycosis: A Comprehensive Review.

Costa R, Hygino da Cruz Jr L, Souza S, Ventura N, Correa D Res Rep Trop Med. 2023; 14:87-98.

PMID: 37554584 PMC: 10406117. DOI: 10.2147/RRTM.S391633.

Immunologic Diagnosis of Endemic Mycoses.

Almeida-Paes R, Bernardes-Engemann A, Motta B, Pizzini C, Almeida M, Muniz M J Fungi (Basel). 2022; 8(10).

PMID: 36294558 PMC: 9605100. DOI: 10.3390/jof8100993.

Paracoccidioidomycosis: Current Perspectives from Brazil.

Mendes R, Cavalcante R, Marques S, Marques M, Venturini J, Sylvestre T Open Microbiol J. 2017; 11:224-282.

PMID: 29204222 PMC: 5695158. DOI: 10.2174/1874285801711010224.

Comparison of virulence between Paracoccidioides brasiliensis and Paracoccidioides lutzii using Galleria mellonella as a host model.

Scorzoni L, de Paula E Silva A, Singulani J, Leite F, de Oliveira H, da Silva R Virulence. 2015; 6(8):766-76.

PMID: 26552324 PMC: 4826127. DOI: 10.1080/21505594.2015.1085277.

References

DuPont B, Huber M, Kim S, Bennett J . Galactomannan antigenemia and antigenuria in aspergillosis: studies in patients and experimentally infected rabbits. J Infect Dis. 1987; 155(1):1-11. DOI: 10.1093/infdis/155.1.1. View

Garcia N, Del Negro G, Martins H, LACAZ C . Detection of paracoccidioidomycosis circulating antigen by the immunoelectroosmophoresis-immunodiffusion technique. Preliminary report. Rev Inst Med Trop Sao Paulo. 1987; 29(5):327-8. DOI: 10.1590/s0036-46651987000500011. View

Bueno J, Shikanai-Yasuda M, Ferreira A, Masuda A . Detection of the 43,000-molecular-weight glycoprotein in sera of patients with paracoccidioidomycosis. J Clin Microbiol. 1989; 27(12):2842-5. PMC: 267138. DOI: 10.1128/jcm.27.12.2842-2845.1989. View

Camargo Z, Unterkircher C, Travassos L . Identification of antigenic polypeptides of Paracoccidioides brasiliensis by immunoblotting. J Med Vet Mycol. 1989; 27(6):407-12. View

Ferreira R, Yu B, Niki Y, Armstrong D . Detection of Candida antigenuria in disseminated candidiasis by immunoblotting. J Clin Microbiol. 1990; 28(5):1075-8. PMC: 267871. DOI: 10.1128/jcm.28.5.1075-1078.1990. View

Giannini M, Bueno J, Shikanai-Yasuda M, Stolf A, Masuda A, AMATO NETO V . Antibody response to the 43 kDa glycoprotein of Paracoccidioides brasiliensis as a marker for the evaluation of patients under treatment. Am J Trop Med Hyg. 1990; 43(2):200-6. DOI: 10.4269/ajtmh.1990.43.200. View

Gomez B, Figueroa J, Hamilton A, Ortiz B, Robledo M, Hay R . Use of monoclonal antibodies in diagnosis of paracoccidioidomycosis: new strategies for detection of circulating antigens. J Clin Microbiol. 1997; 35(12):3278-83. PMC: 230162. DOI: 10.1128/jcm.35.12.3278-3283.1997. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

10.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

11.

Rodrigues M, Cassaguerra C, LACAZ C . Antigenemia in paracoccidioidomycosis. Probable demonstration of circulating antigen by counterimmunoelectrophoresis test. Preliminary report. Rev Inst Med Trop Sao Paulo. 1984; 26(5):285-7. DOI: 10.1590/s0036-46651984000500011. View

12.

Wheat L, Kohler R, Tewari R . Diagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens. N Engl J Med. 1986; 314(2):83-8. DOI: 10.1056/NEJM198601093140205. View

13.

Tuder R, el Ibrahim R, Godoy C, De Brito T . Pathology of the human pulmonary paracoccidioidomycosis. Mycopathologia. 1985; 92(3):179-88. DOI: 10.1007/BF00437631. View

14.

Puccia R, Schenkman S, Gorin P, Travassos L . Exocellular components of Paracoccidioides brasiliensis: identification of a specific antigen. Infect Immun. 1986; 53(1):199-206. PMC: 260097. DOI: 10.1128/iai.53.1.199-206.1986. View

15.

Haynes K, Latge J, Rogers T . Detection of Aspergillus antigens associated with invasive infection. J Clin Microbiol. 1990; 28(9):2040-4. PMC: 268100. DOI: 10.1128/jcm.28.9.2040-2044.1990. View

16.

Camargo Z, Taborda C, Rodrigues E, Travassos L . The use of cell-free antigens of Paracoccidioides brasiliensis in serological tests. J Med Vet Mycol. 1991; 29(1):31-8. View

17.

Roque-Barreira M . Antigenemia in paracoccidioidomycosis. J Clin Microbiol. 1992; 30(2):381-5. PMC: 265064. DOI: 10.1128/jcm.30.2.381-385.1992. View

18.

Moraes R, Ricci T . Proteolytic activity of the 43,000 molecular weight antigen secreted by Paracoccidioides brasiliensis. Rev Inst Med Trop Sao Paulo. 1990; 32(5):384-5. DOI: 10.1590/s0036-46651990000500014. View

19.

Hadad D, Pieres M, Petry T, Orozco S, Melhem M, Paes R . [Paracoccidioides brasiliensis (Lutz, 1908) isolated by hemoculture in a patient with the acquired immunodeficiency syndrome (AIDS)]. Rev Inst Med Trop Sao Paulo. 1992; 34(6):565-7. View

20.

Vaz C, Mackenzie D, Hearn V, Camargo Z, Burger E, Calich V . Gelatinase activity of exoantigens from virulent and non-virulent isolates of Paracoccidioides brasiliensis. J Med Vet Mycol. 1994; 32(1):65-9. DOI: 10.1080/02681219480000091. View