Flow Cytometric Testing of Susceptibilities of Mycobacterium Tuberculosis Isolates to Ethambutol, Isoniazid, and Rifampin in 24 Hours

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1998 Jun 10

PMID 9620378

Citations 14

Authors

S M Kirk

R F Schell

A V MOORE

S M Callister

G H Mazurek

Affiliations

Soon will be listed here.

Abstract

Susceptibility testing of Mycobacterium tuberculosis is seriously limited by the time required to obtain results. We show that susceptibility testing of clinical isolates of M. tuberculosis can be accomplished rapidly with acceptable accuracy by using flow cytometry. The susceptibilities of 35 clinical isolates of M. tuberculosis to various concentrations of isoniazid, rifampin, and ethambutol were tested by the agar proportion method and by flow cytometry. Agreement between the results from the two methods was 95, 92, and 83% for isoniazid, ethambutol, and rifampin, respectively. Only 11 discrepancies were detected among 155 total tests. The results of flow cytometric susceptibility tests were available within 24 h of inoculation of drug-containing medium, while the proportion method required 3 weeks to complete. The flow cytometric method is also simple to perform.

Citing Articles

A Rapid Assessing Method of Drug Susceptibility Using Flow Cytometry for Mycobacterium tuberculosis Isolates Resistant to Isoniazid, Rifampin, and Ethambutol.

Lee S, Baek S, Hong M, Lee J, Cho E, Lee J Tuberc Respir Dis (Seoul). 2022; 85(3):264-272.

PMID: 35196443 PMC: 9263347. DOI: 10.4046/trd.2021.0134.

Revisiting the methods for detecting : what has the new millennium brought thus far?.

Campelo T, de Sousa P, Nogueira L, Frota C, Antas P Access Microbiol. 2021; 3(8):000245.

PMID: 34595396 PMC: 8479963. DOI: 10.1099/acmi.0.000245.

Flow cytometry method for absolute counting and single-cell phenotyping of mycobacteria.

Barr D, Omollo C, Mason M, Koch A, Wilkinson R, Lalloo D Sci Rep. 2021; 11(1):18661.

PMID: 34545154 PMC: 8452731. DOI: 10.1038/s41598-021-98176-5.

Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex.

Tizzano B, Dallenga T, Utpatel C, Behrends J, Homolka S, Kohl T Sci Rep. 2021; 11(1):2628.

PMID: 33514768 PMC: 7846770. DOI: 10.1038/s41598-021-81223-6.

Preclinical Models of Nontuberculous Mycobacteria Infection for Early Drug Discovery and Vaccine Research.

Rampacci E, Stefanetti V, Passamonti F, Henao-Tamayo M Pathogens. 2020; 9(8).

PMID: 32781698 PMC: 7459799. DOI: 10.3390/pathogens9080641.

References

Takayama K, Wang L, MERKAL R . Scanning electron microscopy of the H37Ra strain of Mycobacterium tuberculosis exposed to isoniazid. Antimicrob Agents Chemother. 1973; 4(1):62-5. PMC: 444505. DOI: 10.1128/AAC.4.1.62. View

CANETTI G, Froman S, Grosset J, HAUDUROY P, Langerova M, MAHLER H . MYCOBACTERIA: LABORATORY METHODS FOR TESTING DRUG SENSITIVITY AND RESISTANCE. Bull World Health Organ. 1963; 29:565-78. PMC: 2555065. View

Heifets L, Iseman M, Cook J, DRUPA I . Determination of in vitro susceptibility of Mycobacterium tuberculosis to cephalosporins by radiometric and conventional methods. Antimicrob Agents Chemother. 1985; 27(1):11-5. PMC: 176196. DOI: 10.1128/AAC.27.1.11. View

Siddiqi S, Hawkins J, Laszlo A . Interlaboratory drug susceptibility testing of Mycobacterium tuberculosis by a radiometric procedure and two conventional methods. J Clin Microbiol. 1985; 22(6):919-23. PMC: 271852. DOI: 10.1128/jcm.22.6.919-923.1985. View

Lee C, Heifets L . Determination of minimal inhibitory concentrations of antituberculosis drugs by radiometric and conventional methods. Am Rev Respir Dis. 1987; 136(2):349-52. DOI: 10.1164/ajrccm/136.2.349. View

Nilsson L, Hoffner S, Ansehn S . Rapid susceptibility testing of Mycobacterium tuberculosis by bioluminescence assay of mycobacterial ATP. Antimicrob Agents Chemother. 1988; 32(8):1208-12. PMC: 172378. DOI: 10.1128/AAC.32.8.1208. View

Kawa D, Pennell D, Kubista L, Schell R . Development of a rapid method for determining the susceptibility of Mycobacterium tuberculosis to isoniazid using the Gen-Probe DNA Hybridization System. Antimicrob Agents Chemother. 1989; 33(7):1000-5. PMC: 176052. DOI: 10.1128/AAC.33.7.1000. View

OGorman M, Hopfer R . Amphotericin B susceptibility testing of Candida species by flow cytometry. Cytometry. 1991; 12(8):743-7. DOI: 10.1002/cyto.990120808. View

Jacobs Jr W, Barletta R, Udani R, Chan J, Kalkut G, Sosne G . Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science. 1993; 260(5109):819-22. DOI: 10.1126/science.8484123. View

10.

Gant V, Warnes G, Phillips I, Savidge G . The application of flow cytometry to the study of bacterial responses to antibiotics. J Med Microbiol. 1993; 39(2):147-54. DOI: 10.1099/00222615-39-2-147. View

11.

Ordonez J, Wehman N . Rapid flow cytometric antibiotic susceptibility assay for Staphylococcus aureus. Cytometry. 1993; 14(7):811-8. DOI: 10.1002/cyto.990140714. View

12.

Bloch A, Cauthen G, Onorato I, Dansbury K, Kelly G, Driver C . Nationwide survey of drug-resistant tuberculosis in the United States. JAMA. 1994; 271(9):665-71. View

13.

Mason D, Allman R, Stark J, Lloyd D . Rapid estimation of bacterial antibiotic susceptibility with flow cytometry. J Microsc. 1994; 176(Pt 1):8-16. DOI: 10.1111/j.1365-2818.1994.tb03494.x. View

14.

Raviglione M, Snider Jr D, Kochi A . Global epidemiology of tuberculosis. Morbidity and mortality of a worldwide epidemic. JAMA. 1995; 273(3):220-6. View

15.

Pore R . Antibiotic susceptibility testing by flow cytometry. J Antimicrob Chemother. 1994; 34(5):613-27. DOI: 10.1093/jac/34.5.613. View

16.

Norden M, Kurzynski T, Bownds S, Callister S, Schell R . Rapid susceptibility testing of Mycobacterium tuberculosis (H37Ra) by flow cytometry. J Clin Microbiol. 1995; 33(5):1231-7. PMC: 228136. DOI: 10.1128/jcm.33.5.1231-1237.1995. View

17.

Yajko D, Madej J, Lancaster M, Sanders C, Cawthon V, Gee B . Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis. J Clin Microbiol. 1995; 33(9):2324-7. PMC: 228403. DOI: 10.1128/jcm.33.9.2324-2327.1995. View

18.

Mason D, Gant V . The application of flow cytometry to the estimation of bacterial antibiotic susceptibility. J Antimicrob Chemother. 1995; 36(2):441-3. DOI: 10.1093/jac/36.2.441. View

19.

Durodie J, Coleman K, Simpson I, Loughborough S, Winstanley D . Rapid detection of antimicrobial activity using flow cytometry. Cytometry. 1995; 21(4):374-7. DOI: 10.1002/cyto.990210409. View

20.

. Tuberculosis morbidity--United States, 1995. MMWR Morb Mortal Wkly Rep. 1996; 45(18):365-70. View