Soluble Thrombomodulin and Antibodies to Bovine Glomerular Endothelial Cells in Patients with Henoch-Schönlein Purpura

Overview

Journal Arch Dis Child

Specialty Pediatrics

Date 1998 Jun 5

PMID 9613354

Citations 12

Authors

M Fujieda

N Oishi

K Naruse

M Hashizume

K Nishiya

T Kurashige

K Ito

Affiliations

Soon will be listed here.

Abstract

Aim: To evaluate the clinical significance of soluble thrombomodulin and antiendothelial cell antibodies (AECA) in children with Henoch-Schönlein purpura.

Methods: Binding of serum AECA to bovine glomerular endothelial cells was evaluated by enzyme linked immunosorbent assay, cytotoxicity against glomerular endothelial cells by spectrophotometric assay, and soluble thrombomodulin concentrations by sandwich enzyme immunoassay.

Results: IgA AECA were detected in seven of 15 patients with Henoch-Schönlein purpura and nephritis, but were not detected in patients without nephritis or in controls. Patients with Henoch-Schönlein nephritis had raised titres of IgA AECA and serum thrombomodulin; severe proteinuria and renal histological changes were associated with raised titres of IgA AECA and raised serum thrombomodulin. No subjects had complement dependent cytotoxicity against glomerular endothelial cells.

Conclusions: High titres of IgA AECA and raised serum thrombomodulin may be clinically useful markers of renal involvement in patients with Henoch-Schönlein purpura.

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References

Tan E, Pearson C . Rheumatic disease sera reactive with capillaries in the mouse kidney. Arthritis Rheum. 1972; 15(1):23-8. DOI: 10.1002/art.1780150104. View

Soylemezoglu O, Sultan N, Gursel T, Buyan N, Hasanoglu E . Circulating adhesion molecules ICAM-1, E-selectin, and von Willebrand factor in Henoch-Schönlein purpura. Arch Dis Child. 1996; 75(6):507-11. PMC: 1511812. DOI: 10.1136/adc.75.6.507. View

Cines D, Lyss A, Reeber M, Bina M, DeHoratius R . Presence of complement-fixing anti-endothelial cell antibodies in systemic lupus erythematosus. J Clin Invest. 1984; 73(3):611-25. PMC: 425060. DOI: 10.1172/JCI111251. View

Maruyama I, Bell C, MAJERUS P . Thrombomodulin is found on endothelium of arteries, veins, capillaries, and lymphatics, and on syncytiotrophoblast of human placenta. J Cell Biol. 1985; 101(2):363-71. PMC: 2113687. DOI: 10.1083/jcb.101.2.363. View

Ishii H, MAJERUS P . Thrombomodulin is present in human plasma and urine. J Clin Invest. 1985; 76(6):2178-81. PMC: 424339. DOI: 10.1172/JCI112225. View

Leung D, Collins T, Lapierre L, Geha R, Pober J . Immunoglobulin M antibodies present in the acute phase of Kawasaki syndrome lyse cultured vascular endothelial cells stimulated by gamma interferon. J Clin Invest. 1986; 77(5):1428-35. PMC: 424542. DOI: 10.1172/JCI112454. View

Leung D, Moake J, Havens P, Kim M, Pober J . Lytic anti-endothelial cell antibodies in haemolytic-uraemic syndrome. Lancet. 1988; 2(8604):183-6. DOI: 10.1016/s0140-6736(88)92287-8. View

Rosenbaum J, Pottinger B, Woo P, Black C, Loizou S, Byron M . Measurement and characterisation of circulating anti-endothelial cell IgG in connective tissue diseases. Clin Exp Immunol. 1988; 72(3):450-6. PMC: 1541580. View

Matsuda M . Experimental glomerular tissue injury induced by immunization with cultured endothelial cell plasma membrane. Acta Pathol Jpn. 1988; 38(7):823-39. DOI: 10.1111/j.1440-1827.1988.tb02355.x. View

10.

Suzuki K, Nishioka J, Hayashi T, Kosaka Y . Functionally active thrombomodulin is present in human platelets. J Biochem. 1988; 104(4):628-32. DOI: 10.1093/oxfordjournals.jbchem.a122523. View

11.

Hansen M, Nielsen S, Berg K . Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods. 1989; 119(2):203-10. DOI: 10.1016/0022-1759(89)90397-9. View

12.

Iwashima Y, Sato T, Watanabe K, Ooshima E, Hiraishi S, Ishii H . Elevation of plasma thrombomodulin level in diabetic patients with early diabetic nephropathy. Diabetes. 1990; 39(8):983-8. DOI: 10.2337/diab.39.8.983. View

13.

Quadros N, Roberts-Thomson P, Gallus A . IgG and IgM anti-endothelial cell antibodies in patients with collagen-vascular disorders. Rheumatol Int. 1990; 10(3):113-9. DOI: 10.1007/BF02274825. View

14.

Frampton G, Jayne D, Perry G, Lockwood C, Cameron J . Autoantibodies to endothelial cells and neutrophil cytoplasmic antigens in systemic vasculitis. Clin Exp Immunol. 1990; 82(2):227-32. PMC: 1535114. DOI: 10.1111/j.1365-2249.1990.tb05431.x. View

15.

Tanaka A, Ishii H, Hiraishi S, Kazama M, Maezawa H . Increased thrombomodulin values in plasma of diabetic men with microangiopathy. Clin Chem. 1991; 37(2):269-72. View

16.

Kodama S, Uchijima E, Nagai M, Mikawatani K, Hayashi T, Suzuki K . One-step sandwich enzyme immunoassay for soluble human thrombomodulin using monoclonal antibodies. Clin Chim Acta. 1990; 192(3):191-9. DOI: 10.1016/0009-8981(90)90221-d. View

17.

Ishii H, Uchiyama H, Kazama M . Soluble thrombomodulin antigen in conditioned medium is increased by damage of endothelial cells. Thromb Haemost. 1991; 65(5):618-23. View

18.

Nitta K, Simonson M, Dunn M . The regulation and role of prostaglandin biosynthesis in cultured bovine glomerular endothelial cells. J Am Soc Nephrol. 1991; 2(2):156-63. DOI: 10.1681/ASN.V22156. View

19.

Del Papa N, Meroni P, Barcellini W, Sinico A, Radice A, Tincani A . Antibodies to endothelial cells in primary vasculitides mediate in vitro endothelial cytotoxicity in the presence of normal peripheral blood mononuclear cells. Clin Immunol Immunopathol. 1992; 63(3):267-74. DOI: 10.1016/0090-1229(92)90232-d. View

20.

Ohdama S, Takano S, Miyake S, Kubota T, Sato K, Aoki N . Plasma thrombomodulin as a marker of vascular injuries in collagen vascular diseases. Am J Clin Pathol. 1994; 101(1):109-13. DOI: 10.1093/ajcp/101.1.109. View